Investigation of Break-Induced Replication in Yeast

Beth Osia, Rajula Elango, Juraj Kramara, Steven A. Roberts, Anna Malkova

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Repair of double-strand DNA breaks (DSBs) is important for preserving genomic integrity and stability. Break-induced replication (BIR) is a mechanism aimed to repair one-ended double-strand DNA breaks, similar to those formed by replication fork collapse or by telomere erosion. Unlike S-phase replication, BIR is carried out by a migrating DNA bubble and is associated with conservative inheritance of newly synthesized DNA. This unusual DNA synthesis leads to high level of mutagenesis and chromosomal rearrangements during BIR. Here, we focus on several genetic and molecular methods to investigate BIR using our system in yeast Saccharomyces cerevisiae where BIR is initiated by a site-specific DNA break, and the repair involves two copies of chromosome III.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages307-328
Number of pages22
DOIs
StatePublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2153
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • APOBEC
  • Break-induced replication
  • Contour-clamped homogenous electric field electrophoresis
  • Double-strand break
  • Gross chromosomal rearrangements
  • Homologous recombination
  • Single-stranded DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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