Intratracheal inoculation of Fischer 344 rats with Francisella tularensis

Jesse Q. Nguyen; Xhavit Zogaj; Aanuoluwa A. Adelani; Ping Chu; Jieh Juen Yu; Bernard P. Arulanandam; Karl E. Klose

doi:10.3791/56123

Intratracheal inoculation of Fischer 344 rats with Francisella tularensis

Jesse Q. Nguyen, Xhavit Zogaj, Aanuoluwa A. Adelani, Ping Chu, Jieh Juen Yu, Bernard P. Arulanandam, Karl E. Klose

Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Pulmonary infection with the bacterium Francisella tularensis can lead to the serious and potentially fatal disease, tularemia, in humans. Due to the current lack of an approved tularemia vaccine for humans, research is focused on vaccine development utilizing appropriate animal models. The Fischer 344 rat has emerged as a model that reflects human susceptibility to F. tularensis infection, and thus is an attractive model for tularemia vaccine development. Intratracheal inoculation of the Fischer 344 rat with F. tularensis mimics pulmonary exposure in humans. The successful delivery into the rat trachea is critical for pulmonary delivery. A laryngoscope with illumination is used to properly intubate the tracheae of anesthetized rats; the correct placement within the trachea is determined by a simple device to detect breathing. Following intubation, the F. tularensis culture is delivered in a measured dose via syringe. This technique standardizes pulmonary delivery of F. tularensis within the rat trachea to evaluate vaccine efficacy.

Original language	English (US)
Article number	e56123
Journal	Journal of Visualized Experiments
Volume	2017
Issue number	127
DOIs	https://doi.org/10.3791/56123
State	Published - Sep 30 2017

Keywords

Biothreat
Francisella
Immunology
Intratracheal
Issue 127
Rat
Tularemia
Vaccine

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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title = "Intratracheal inoculation of Fischer 344 rats with Francisella tularensis",

abstract = "Pulmonary infection with the bacterium Francisella tularensis can lead to the serious and potentially fatal disease, tularemia, in humans. Due to the current lack of an approved tularemia vaccine for humans, research is focused on vaccine development utilizing appropriate animal models. The Fischer 344 rat has emerged as a model that reflects human susceptibility to F. tularensis infection, and thus is an attractive model for tularemia vaccine development. Intratracheal inoculation of the Fischer 344 rat with F. tularensis mimics pulmonary exposure in humans. The successful delivery into the rat trachea is critical for pulmonary delivery. A laryngoscope with illumination is used to properly intubate the tracheae of anesthetized rats; the correct placement within the trachea is determined by a simple device to detect breathing. Following intubation, the F. tularensis culture is delivered in a measured dose via syringe. This technique standardizes pulmonary delivery of F. tularensis within the rat trachea to evaluate vaccine efficacy.",

keywords = "Biothreat, Francisella, Immunology, Intratracheal, Issue 127, Rat, Tularemia, Vaccine",

author = "Nguyen, {Jesse Q.} and Xhavit Zogaj and Adelani, {Aanuoluwa A.} and Ping Chu and Yu, {Jieh Juen} and Arulanandam, {Bernard P.} and Klose, {Karl E.}",

note = "Funding Information: This study was supported by the Defense Threat Reduction Agency (DTRA) under contract HDTRA1-14-C-0116, and the Center for Excellence in Infection Genomics (DOD #W911NF-11-1-0136).",

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AU - Nguyen, Jesse Q.

AU - Zogaj, Xhavit

AU - Adelani, Aanuoluwa A.

AU - Chu, Ping

AU - Yu, Jieh Juen

AU - Arulanandam, Bernard P.

AU - Klose, Karl E.

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N2 - Pulmonary infection with the bacterium Francisella tularensis can lead to the serious and potentially fatal disease, tularemia, in humans. Due to the current lack of an approved tularemia vaccine for humans, research is focused on vaccine development utilizing appropriate animal models. The Fischer 344 rat has emerged as a model that reflects human susceptibility to F. tularensis infection, and thus is an attractive model for tularemia vaccine development. Intratracheal inoculation of the Fischer 344 rat with F. tularensis mimics pulmonary exposure in humans. The successful delivery into the rat trachea is critical for pulmonary delivery. A laryngoscope with illumination is used to properly intubate the tracheae of anesthetized rats; the correct placement within the trachea is determined by a simple device to detect breathing. Following intubation, the F. tularensis culture is delivered in a measured dose via syringe. This technique standardizes pulmonary delivery of F. tularensis within the rat trachea to evaluate vaccine efficacy.

AB - Pulmonary infection with the bacterium Francisella tularensis can lead to the serious and potentially fatal disease, tularemia, in humans. Due to the current lack of an approved tularemia vaccine for humans, research is focused on vaccine development utilizing appropriate animal models. The Fischer 344 rat has emerged as a model that reflects human susceptibility to F. tularensis infection, and thus is an attractive model for tularemia vaccine development. Intratracheal inoculation of the Fischer 344 rat with F. tularensis mimics pulmonary exposure in humans. The successful delivery into the rat trachea is critical for pulmonary delivery. A laryngoscope with illumination is used to properly intubate the tracheae of anesthetized rats; the correct placement within the trachea is determined by a simple device to detect breathing. Following intubation, the F. tularensis culture is delivered in a measured dose via syringe. This technique standardizes pulmonary delivery of F. tularensis within the rat trachea to evaluate vaccine efficacy.

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