Intratracheal inoculation of Fischer 344 rats with Francisella tularensis

Jesse Q. Nguyen, Xhavit Zogaj, Aanuoluwa A. Adelani, Ping Chu, Jieh Juen Yu, Bernard P. Arulanandam, Karl E. Klose

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Pulmonary infection with the bacterium Francisella tularensis can lead to the serious and potentially fatal disease, tularemia, in humans. Due to the current lack of an approved tularemia vaccine for humans, research is focused on vaccine development utilizing appropriate animal models. The Fischer 344 rat has emerged as a model that reflects human susceptibility to F. tularensis infection, and thus is an attractive model for tularemia vaccine development. Intratracheal inoculation of the Fischer 344 rat with F. tularensis mimics pulmonary exposure in humans. The successful delivery into the rat trachea is critical for pulmonary delivery. A laryngoscope with illumination is used to properly intubate the tracheae of anesthetized rats; the correct placement within the trachea is determined by a simple device to detect breathing. Following intubation, the F. tularensis culture is delivered in a measured dose via syringe. This technique standardizes pulmonary delivery of F. tularensis within the rat trachea to evaluate vaccine efficacy.

Original languageEnglish (US)
Article numbere56123
JournalJournal of Visualized Experiments
Volume2017
Issue number127
DOIs
StatePublished - Sep 30 2017

Keywords

  • Biothreat
  • Francisella
  • Immunology
  • Intratracheal
  • Issue 127
  • Rat
  • Tularemia
  • Vaccine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Intratracheal inoculation of Fischer 344 rats with Francisella tularensis'. Together they form a unique fingerprint.

Cite this