It is clear from the histologic studies that tissue contained in the Nucleopore chambers is accessible to cellular infiltration by host cells. Furthermore, this model enables one to track the sequence of infiltration and allows histologic evaluation of the damage caused by the infiltrating cells in a relatively well preserved piece of organ-specific tissue. The fact that cells appear to come and go indicates that this is a dynamic model that allows us to study cells retrieved during their transit period while they are in the fluid surrounding the graft. Thus, extensive extraction of the cells from the invaded tissue are not required. Although there was no significant evidence of spontaneous specific cytotoxicity detectable within the limitations of this current study, we predict that further studies of the cells retrieved from chambers bearing allogeneic tissue will demonstrate a primed secondary response when challenged with alloantigen in vitro prior to testing for cytotoxicity. Phenotypic analysis of the infiltrating cells is as yet incomplete but data strongly suggest that indeed the null cell population appears to be involved in the host response to allogeneic tissue. The marked increase in ADA activity seen in the cells infiltrating our allogeneic model is consistent with our other observations that high ADA activity in mononuclear cells is associated with immune responsiveness in the organ allograft setting. We conclude that the Nucleopore chamber allograft model shows promise for use as a tool to study the cells infiltrating rejecting tissue while providing retrieval of the cells with minimal trauma and greatest likelihood of exhibiting metabolic processes closest to those occurring in vivo.
|Original language||English (US)|
|Number of pages||3|
|State||Published - 1988|
ASJC Scopus subject areas