Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

Yu Cong; Madhulika Jupelli; M. Neal Guentzel; Guangming Zhong; Ashlesh K. Murthy; Bernard P. Arulanandam

doi:10.1016/j.vaccine.2007.02.010

Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

Yu Cong
, Madhulika Jupelli
, M. Neal Guentzel
, Guangming Zhong
, Ashlesh K. Murthy
, Bernard P. Arulanandam

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

Original language	English (US)
Pages (from-to)	3773-3780
Number of pages	8
Journal	Vaccine
Volume	25
Issue number	19
DOIs	https://doi.org/10.1016/j.vaccine.2007.02.010
State	Published - May 10 2007

Keywords

Adjuvant
CPAF
Chlamydia
Chlamydial protease-like activity factor
CpG
Genital
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/j.vaccine.2007.02.010

Cite this

@article{a1c297e0f7914558a851e6359f00e6f2,

title = "Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection",

abstract = "We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.",

keywords = "Adjuvant, CPAF, Chlamydia, Chlamydial protease-like activity factor, CpG, Genital, Vaccine",

author = "Yu Cong and Madhulika Jupelli and Guentzel, \{M. Neal\} and Guangming Zhong and Murthy, \{Ashlesh K.\} and Arulanandam, \{Bernard P.\}",

note = "Funding Information: This work was supported by National Institutes of Health grants AR048973 and GM08194. The authors thank Dr. Jieh-Juen Yu for critical review of the manuscript.",

year = "2007",

month = may,

day = "10",

doi = "10.1016/j.vaccine.2007.02.010",

language = "English (US)",

volume = "25",

pages = "3773--3780",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier Ltd",

number = "19",

}

TY - JOUR

T1 - Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

AU - Cong, Yu

AU - Jupelli, Madhulika

AU - Guentzel, M. Neal

AU - Zhong, Guangming

AU - Murthy, Ashlesh K.

AU - Arulanandam, Bernard P.

N1 - Funding Information: This work was supported by National Institutes of Health grants AR048973 and GM08194. The authors thank Dr. Jieh-Juen Yu for critical review of the manuscript.

PY - 2007/5/10

Y1 - 2007/5/10

N2 - We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

AB - We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

KW - Adjuvant

KW - CPAF

KW - Chlamydia

KW - Chlamydial protease-like activity factor

KW - CpG

KW - Genital

KW - Vaccine

UR - https://www.scopus.com/pages/publications/34247163593

UR - https://www.scopus.com/pages/publications/34247163593#tab=citedBy

U2 - 10.1016/j.vaccine.2007.02.010

DO - 10.1016/j.vaccine.2007.02.010

M3 - Article

C2 - 17349723

AN - SCOPUS:34247163593

SN - 0264-410X

VL - 25

SP - 3773

EP - 3780

JO - Vaccine

JF - Vaccine

IS - 19

ER -

Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this