Intranasal immunization with chlamydial protease-like activity factor and CpG deoxynucleotides enhances protective immunity against genital Chlamydia muridarum infection

Yu Cong, Madhulika Jupelli, M. Neal Guentzel, Guangming Zhong, Ashlesh K. Murthy, Bernard P. Arulanandam

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.

Original languageEnglish (US)
Pages (from-to)3773-3780
Number of pages8
JournalVaccine
Volume25
Issue number19
DOIs
StatePublished - May 10 2007

Keywords

  • Adjuvant
  • CPAF
  • Chlamydia
  • Chlamydial protease-like activity factor
  • CpG
  • Genital
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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