Abstract
We have reported recently that intranasal (i.n.) vaccination with chlamydial protease-like activity factor (CPAF) and interleukin-12 (IL-12) enhances protective immunity against genital chlamydial challenge. In this study, we show that i.n. or intraperitoneal (i.p.) vaccination with CPAF plus CpG deoxynucleotides (CpG), an alternative T helper 1 (Th1) adjuvant, induced robust CPAF-specific IFN-γ responses and elevated levels of serum antibody and vaginal IgA production. CPAF + CpG vaccinated animals displayed accelerated genital chlamydial clearance, and minimal hydrosalpinx and inflammatory cellular infiltration compared to mock-immunized (PBS) challenged animals. Together, CpG dexoynucleotides are an efficacious alternative Th1 adjuvant with CPAF to induce protective anti-chlamydial immunity.
Original language | English (US) |
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Pages (from-to) | 3773-3780 |
Number of pages | 8 |
Journal | Vaccine |
Volume | 25 |
Issue number | 19 |
DOIs | |
State | Published - May 10 2007 |
Keywords
- Adjuvant
- CPAF
- Chlamydia
- Chlamydial protease-like activity factor
- CpG
- Genital
- Vaccine
ASJC Scopus subject areas
- Molecular Medicine
- General Immunology and Microbiology
- General Veterinary
- Public Health, Environmental and Occupational Health
- Infectious Diseases