Intracoronary photodynamic therapy reduces neointimal growth without suppressing re-endothelialisation in a porcine model

Ron Waksman, I. M. Leitch, J. Roessler, H. Yazdi, R. Seabron, F. Tio, R. W. Scott, R. I. Grove, S. Rychnovsky, B. Robinson, R. Pakala, E. Cheneau

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Objective: To examine the effects of intracoronary PhotoPoint photodynamic therapy (PDT) with a new photosensitiser, MV0611, in the overstretch balloon and stent porcine models of restenosis. Methods: 28 pigs were injected with 3 mg/kg of MV0611 systemically 4 h before the procedure. Animals were divided into either the balloon overstretch injury (BI) group (n = 19) or the stented group (n = 9). After BI, a centred delivery catheter was positioned in the artery to cover the injured area, and light (532 nm, 125 J/cm2) was applied to activate the drug (n = 10). Control arteries (n = 9) were not activated by light. In the stented group, the drug was light activated before stent deployment. Serial sections of vessels were processed 14 days after treatment in the BI group and 30 days after treatment in the stented group for histomorphometric or immunohistochemical analysis. Results: Intracoronary PDT significantly reduced intimal thickness in both BI and stented arteries (about 65%: 0.22 (SEM 0.05) mm v 0.62 (0.05) mm, p < 0.01; and about 26%: 0.40 (0.04) mm v 0.54 (0.04) mm, p < 0.01, respectively). PDT increased luminal area by ≤ 60% and 50% within BI and stented arteries (3.43 (0.27) mm 2 v 5.51 (0.52) mm2, p < 0.05; 4.0 (0.02) mm 2 v 6.0 (0.16) mm2, p < 0.01), respectively. Complete re-endothelialisation was observed by immunohistochemical and gross histological analyses in all PDT and control arteries. There were no cases of aneurysm formation or thrombosis. Conclusion: Intracoronary PhotoPoint PDT with MV0611 reduces intimal proliferation without suppressing re-endothelialisation in a porcine model of restenosis.

Original languageEnglish (US)
Pages (from-to)1138-1144
Number of pages7
JournalHeart
Volume92
Issue number8
DOIs
StatePublished - Aug 2006
Externally publishedYes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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