Intrachromosomal genomic instability in human sporadic colorectal cancer measured by genome-wide allelotyping and inter-(simple sequence repeat) PCR

Garth R. Anderson, W. Michael Henry, Jeffrey M. Conroy, Matthew J. Karpenko, Neng Chen, Jeremy D. Bartos, Joslyn K. Brunelle, Gerald P. Jahreis, Norma J. Nowak, Thomas B. Shows, Garth R. Anderson, Bruce M. Brenner, Miguel A. Rodriguez-Bigas, Nicholas J. Petrelli, Helen Swede, Sheila Sait, Daniel L. Stoler, Jean Pierre Issa, Morton S. Kahlenberg, Mark Basik

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

We have used genome-wide allelotyping with 348 polymorphic autosomal markers spaced, on average, 10 cM apart to quantitate the extent of intrachromosomal instability in 59 human sporadic colorectal carcinomas. We have compared instability measured by this method with that measured by inter-(simple sequence repeat) PCR and microsatellite instability assays. Instability quantitated by fractional allelic loss rates was found to be independent of that detected by microsatellite instability analyses but was weakly associated with that measured by inter-(simple sequence repeat) PCR. A set of seven loci were identified that were most strongly associated with elevated rates of fractional allelic loss and/or inter-(simple sequence repeat) PCR instability; these seven loci were on chromosomes 3, 8, 11, 13, 14, 18, and 20. A lesser association was seen with two loci flanking p53 on chromosome 17. Coordinate loss patterns for these loci suggest that at least two separate sets of cooperating loci exist for intrachromosomal genomic instability in human colorectal cancer.

Original languageEnglish (US)
Pages (from-to)8274-8283
Number of pages10
JournalCancer Research
Volume61
Issue number22
StatePublished - Nov 15 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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