Intracerebral administration of neuronal nitric oxide synthase antiserum attenuates traumatic brain injury-induced blood-brain barrier permeability, brain edema formation, and sensory motor disturbances in the rat

Hari S. Sharma, L. Wiklund, R. D. Badgaiyan, S. Mohanty, P. Alm

Research output: Chapter in Book/Report/Conference proceedingChapter

33 Scopus citations

Abstract

The role of nitric oxide (NO) in traumatic brain injury (TBI)-induced sensory motor function and brain pathology was examined using intracerebral administration of neuronal nitric oxide synthase (nNOS) antiserum in a rat model. TBI was produced by a making a longitudinal incision into the right parietal cerebral cortex limited to the dorsal surface of the hippocampus. Focal TBI induces profound edematous swelling, extravasation of Evans blue dye, and up-regulation of nNOS in the injured cerebral cortex and the underlying subcortical areas at 5 hours. The traumatized animals exhibited pronounced sensory motor deficit, as seen using Rota-Rod and grid-walking tests. Intracerebral administration of nNOS antiserum (1 : 20) 5 minutes and 1 hour after TBI significantly attenuated brain edema formation, Evans blue leakage, and nNOS expression in the injured cortex and the underlying subcortical regions. The nNOS antiserum-treated rats showed improved sensory motor functions. However, administration of nNOS antiserum 2 hours after TBI did not influence these parameters significantly. These novel observations suggest that NO participates in blood-brain barrier disruption, edema formation, and sensory motor disturbances in the early phase of TBI, and that nNOS antiserum has some potential therapeutic value requiring additional investigation.

Original languageEnglish (US)
Title of host publicationBrain Edema XIII
PublisherSpringer-Verlag Wien
Pages288-294
Number of pages7
Edition96
ISBN (Print)9783211307120
DOIs
StatePublished - 2006
Externally publishedYes

Publication series

NameActa Neurochirurgica, Supplementum
Number96
ISSN (Print)0065-1419

Keywords

  • blood-brain barrier
  • edema
  • neuronal nitric oxide synthase
  • nitric oxide
  • sensory motor functions
  • Traumatic brain injury

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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