Intracellular transport of SV40 large tumor antigen: A mutation which abolishes migration to the nucleus does not prevent association with the cell surface

Robert E. Lanford, Janet S. Butel

Research output: Contribution to journalArticle

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Abstract

The cT mutation of PARA, an SV40-adenovirus 7 hybrid virus, abolishes the transport of SV40 T-antigen (T-ag) to the nucleus and results in the accumulation of T-ag in the cytoplasm of infected and transformed cells. The effect of the cT mutation on the association of T-ag with the cell surface was examined in PARA(cT)-infected monkey cells. Surface-associated T-ag was detected by immunofluorescence,lactoperoxidase-catalyzed cell-surface iodination, and cellular fractionation. Surface-associated T-ag was more readily labeled by cell-surface iodination with PARA(cT)-infected cells than with SV40 and wild-type PARA [PARA(nT)]-infected cells. Control experiments eliminated the possibility that the enhanced detection of T-ag in PARA(cT)-infected cells was due to the labeling of intracellular T-ag by lactoperoxidase or to T-ag leaking from dead cells and adhering to the cell surface. The elevated level of surface-associated T-ag in PARA(cT)-infected cells was confirmed by metabolic labeling and cellular fractionation, indicating that the increased iodination of surface-associated T-ag was due to a greater number of T-ag polypeptides on the surface rather than a greater exposure of tyrosine residues. Pulse-chase experiments demonstrated that the elevated level of surface-associated T-ag in PARA(cT)-infected cells was not due to a prolonged stability of surface-associated T-ag. The cT mutation provides a useful tool for studies of mechanisms governing intracellular protein transport.

Original languageEnglish (US)
Pages (from-to)169-184
Number of pages16
JournalVirology
Volume119
Issue number1
DOIs
StatePublished - 1982
Externally publishedYes

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Viral Tumor Antigens
Neoplasm Antigens
Mutation
Halogenation
Lactoperoxidase
Polyomavirus Transforming Antigens
Protein Transport
Adenoviridae
Haplorhini
Fluorescent Antibody Technique
Tyrosine
Cytoplasm
Viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Intracellular transport of SV40 large tumor antigen : A mutation which abolishes migration to the nucleus does not prevent association with the cell surface. / Lanford, Robert E.; Butel, Janet S.

In: Virology, Vol. 119, No. 1, 1982, p. 169-184.

Research output: Contribution to journalArticle

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