Intracellular delivery of p53 fused to the basic domain of HIV-1 Tat

Jiyoon Ryu, Hak Joo Lee, Kyeong Ae Kim, Jae Yong Lee, Kil Soo Lee, Jinseu Park, Soo Young Choi

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


p53 is a potent tumor suppressor inactivated in many cancers. In this study, the membrane permeability of the HIV-1 Tat basic domain was exploited to introduce functional p53 into cancer cells. We expressed and purified a p53 fusion protein with the HIV-1 Tat basic domain at its N terminus (Tat-p53), and examined its transduction profile and biological activity in cancer cells. Tat-p53 was efficiently delivered to both the cytoplasm and nucleus of cells, and was transcriptionally active, as judged by the level of p21/WAF1 protein and of p21 promoter activity. Transduction of cells with Tat-p53 resulted in apoptotic cell death in both p53 positive and negative human tumor cell lines. These results suggest that Tat-p53 could be useful in cancer therapy.

Original languageEnglish (US)
Pages (from-to)353-359
Number of pages7
JournalMolecules and Cells
Issue number2
StatePublished - Apr 30 2004
Externally publishedYes


  • Apoptosis
  • Cancer therapy
  • Tat
  • Transduction
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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