Interphase FISH screening for the LCR-mediated common rearrangement of isochromosome 17q in primary myelofibrosis

Gabriel A. Bien-Willner, Paweł Stankiewicz, James R. Lupski, Jill K. Northup, Gopalrao V Velagaleti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Non-allelic homologous recombination (NAHR) between low-copy repeats (LCRs) has been implicated recently in somatic rearrangements including isochromosome i(17q), which is associated with hematologic malignancies as well as solid tumors. In hematological malignancies, the most common i(17q) breakpoint results from LCR-mediated NAHR, which creates a dicentric chromosome, idic(17)(p11.2). We report an elderly patient who presented with primary myelofibrosis (MF) with myeloid metaplasia (MMM), associated with idic(17)(p11.2) as the sole chromosomal abnormality, making this the first idic(17)(p11.2) myeloproliferative case reported in which the breakpoints are mapped to the breakpoint cluster region in proximal 17p. The rearrangement breakpoint maps to the previously defined LCR cluster, further suggesting that the genomic architecture of proximal 17p may be responsible for the formation of the majority of i(17q) cases. We describe our development of a rapid screening test using interphase FISH to detect idic(17)(p11.2), discuss the potential prognostic value of this molecular diagnostic test, and examine the relevance of LCR-mediated NAHR to common rearrangements in neoplasms.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalAmerican Journal of Hematology
Volume79
Issue number4
DOIs
StatePublished - Aug 2005
Externally publishedYes

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Genomic Segmental Duplications
Isochromosomes
Primary Myelofibrosis
Interphase
Homologous Recombination
Hematologic Neoplasms
Chromosomes, Human, Pair 17
Molecular Pathology
Routine Diagnostic Tests
Chromosome Aberrations
Neoplasms

ASJC Scopus subject areas

  • Hematology

Cite this

Interphase FISH screening for the LCR-mediated common rearrangement of isochromosome 17q in primary myelofibrosis. / Bien-Willner, Gabriel A.; Stankiewicz, Paweł; Lupski, James R.; Northup, Jill K.; Velagaleti, Gopalrao V.

In: American Journal of Hematology, Vol. 79, No. 4, 08.2005, p. 309-313.

Research output: Contribution to journalArticle

Bien-Willner, Gabriel A. ; Stankiewicz, Paweł ; Lupski, James R. ; Northup, Jill K. ; Velagaleti, Gopalrao V. / Interphase FISH screening for the LCR-mediated common rearrangement of isochromosome 17q in primary myelofibrosis. In: American Journal of Hematology. 2005 ; Vol. 79, No. 4. pp. 309-313.
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