International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices

Lora W. Barsky, Michele Black, Matthew Cochran, Benjamin J. Daniel, Derek Davies, Monica DeLay, Rui Gardner, Michael Gregory, Desiree Kunkel, Joanne Lannigan, James Marvin, Robert Salomon, Carina Torres, Rachael Walker

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10 Scopus citations

Abstract

The purpose of this document is to define minimal standards for a flow cytometry shared resource laboratory (SRL) and provide guidance for best practices in several important areas. This effort is driven by the desire of International Society for the Advancement of Cytometry (ISAC) members in SRLs to define and maintain standards of excellence in flow cytometry, and act as a repository for key elements of this information (e.g. example SOPs/training material, etc.). These best practices are not intended to define specifically how to implement these recommendations, but rather to establish minimal goals for an SRL to address in order to achieve excellence. It is hoped that once these best practices are established and implemented they will serve as a template from which similar practices can be defined for other types of SRLs. Identification of the need for best practices first occurred through discussions at the CYTO 2013 SRL Forum, with the most important areas for which best practices should be defined identified through several surveys and SRL track workshops as part of CYTO 2014.

Original languageEnglish (US)
Pages (from-to)1017-1030
Number of pages14
JournalCytometry Part A
Volume89
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

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Keywords

  • best practices
  • biosafety
  • data management
  • education and training
  • flow cytometry shared resource
  • operations management
  • quality assurance
  • quality control
  • reproducibility in science
  • standard operating procedures

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Barsky, L. W., Black, M., Cochran, M., Daniel, B. J., Davies, D., DeLay, M., ... Walker, R. (2016). International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices. Cytometry Part A, 89(11), 1017-1030. https://doi.org/10.1002/cyto.a.23016