Abstract
Purpose: It has been proposed that cytokine gene polymorphisms can predispose individuals to disease by enhancing inflammatory processes. Considering the relevance of interleukin-I (IL-1) in the pathogenesis of toxoplasmic retinochoroiditis (TR), we investigated whether IL1A -889 C/ T and IL1B +3954C/T promoter polymorphisms are associated with TR in humans. Methods: We performed cross-sectional study that involved 100 Brazilian TR patients and 100 age- and gender-matched control subjects. Genomic DNA was obtained from oral swabs of all participants and amplified using polymerase chain reaction (PCR) with specific primers flanking the locus -889 of IL1A and +3954 of IL1B. PCR products were submitted to digestion and analyzed by PAGE to distinguish C and T alleles. Results: There was no significant difference in the genotype or allele distributions of the IL1A -889 C/T and IL1B +3954C/T polymorphisms in patients with TR when compared with controls. However, in a subgroup analysis, the frequency of genotype and allele distributions of IL1A -889 C/T differed significantly between TR patients with and without recurrent episodes. Conclusion: This study suggests that the genotypes related with a high production of IL-1a may be associated with the recurrence of TR.
Original language | English (US) |
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Pages (from-to) | 1845-1849 |
Number of pages | 5 |
Journal | Molecular vision |
Volume | 14 |
State | Published - Oct 12 2008 |
Externally published | Yes |
ASJC Scopus subject areas
- Ophthalmology