Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst; S. E. Kidd; C. O. Morrissey; E. Snelders; W. J.G. Melchers; M. V. Castelli; E. Mellado; K. Simmon; C. A. Petti; S. Richardson; S. Zhang; A. M. Romanelli; B. L. Wickes; H. A. De Valk; C. H.W. Klaassen; S. Arunmozhi Balajee

doi:10.1128/JCM.00124-09

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S. F. Hurst, S. E. Kidd, C. O. Morrissey, E. Snelders, W. J.G. Melchers, M. V. Castelli, E. Mellado, K. Simmon, C. A. Petti, S. Richardson, S. Zhang, A. M. Romanelli, B. L. Wickes, H. A. De Valk, C. H.W. Klaassen, S. Arunmozhi Balajee

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original language	English (US)
Pages (from-to)	1562-1564
Number of pages	3
Journal	Journal of clinical microbiology
Volume	47
Issue number	5
DOIs	https://doi.org/10.1128/JCM.00124-09
State	Published - May 2009

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/JCM.00124-09

Cite this

Hurst, S. F., Kidd, S. E., Morrissey, C. O., Snelders, E., Melchers, W. J. G., Castelli, M. V., Mellado, E., Simmon, K., Petti, C. A., Richardson, S., Zhang, S., Romanelli, A. M., Wickes, B. L., De Valk, H. A., Klaassen, C. H. W., & Arunmozhi Balajee, S. (2009). Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus. Journal of clinical microbiology, 47(5), 1562-1564. https://doi.org/10.1128/JCM.00124-09

Hurst, SF, Kidd, SE, Morrissey, CO, Snelders, E, Melchers, WJG, Castelli, MV, Mellado, E, Simmon, K, Petti, CA, Richardson, S, Zhang, S, Romanelli, AM, Wickes, BL, De Valk, HA, Klaassen, CHW & Arunmozhi Balajee, S 2009, 'Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus', Journal of clinical microbiology, vol. 47, no. 5, pp. 1562-1564. https://doi.org/10.1128/JCM.00124-09

@article{00394c62785c4d07881987e3b2ed5e11,

title = "Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus",

abstract = "Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.",

author = "Hurst, {S. F.} and Kidd, {S. E.} and Morrissey, {C. O.} and E. Snelders and Melchers, {W. J.G.} and Castelli, {M. V.} and E. Mellado and K. Simmon and Petti, {C. A.} and S. Richardson and S. Zhang and Romanelli, {A. M.} and Wickes, {B. L.} and {De Valk}, {H. A.} and Klaassen, {C. H.W.} and {Arunmozhi Balajee}, S.",

year = "2009",

month = may,

doi = "10.1128/JCM.00124-09",

language = "English (US)",

volume = "47",

pages = "1562--1564",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

AU - Hurst, S. F.

AU - Kidd, S. E.

AU - Morrissey, C. O.

AU - Snelders, E.

AU - Melchers, W. J.G.

AU - Castelli, M. V.

AU - Mellado, E.

AU - Simmon, K.

AU - Petti, C. A.

AU - Richardson, S.

AU - Zhang, S.

AU - Romanelli, A. M.

AU - Wickes, B. L.

AU - De Valk, H. A.

AU - Klaassen, C. H.W.

AU - Arunmozhi Balajee, S.

PY - 2009/5

Y1 - 2009/5

N2 - Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

AB - Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

UR - http://www.scopus.com/inward/record.url?scp=66149117086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66149117086&partnerID=8YFLogxK

U2 - 10.1128/JCM.00124-09

DO - 10.1128/JCM.00124-09

M3 - Article

C2 - 19261791

AN - SCOPUS:66149117086

SN - 0095-1137

VL - 47

SP - 1562

EP - 1564

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 5

ER -

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this