Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence

Paul Brehm; James Lechleiter; Stephen Smith; Kathleen Dunlap

doi:10.1016/0896-6273(89)90032-9

Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence

Paul Brehm, James Lechleiter, Stephen Smith, Kathleen Dunlap

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Bioluminescence in the hydrozoan coelenterate Obelia results from calcium activation of a photoprotein contained in light-emitting cells (photocytes) scattered in the animal's endoderm. The influx of calcium into non-luminescent endodermal cells through conventional voltage-dependent calcium channels is required for the excitation-luminescence coupling. Our results suggest that the subsequent diffusion of this calcium, via gap junctions, into the neighboring photocytes triggers a localized luminescence response. Following intense stimulation, the local rise in calcium elicits a secondary wave of luminescence that is supported by a voltage-independent calcium permeability mechanism in the photocyte plasma membrane. These two mechanisms for elevating internal calcium in light-emitting cells can account for the spatial and temporal features of intra-cellular luminescence in Obelia.

Original language	English (US)
Pages (from-to)	191-198
Number of pages	8
Journal	Neuron
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/0896-6273(89)90032-9
State	Published - Aug 1989
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0896-6273(89)90032-9

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@article{d12e55cc00254b2496d10edc25022705,

title = "Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence",

abstract = "Bioluminescence in the hydrozoan coelenterate Obelia results from calcium activation of a photoprotein contained in light-emitting cells (photocytes) scattered in the animal's endoderm. The influx of calcium into non-luminescent endodermal cells through conventional voltage-dependent calcium channels is required for the excitation-luminescence coupling. Our results suggest that the subsequent diffusion of this calcium, via gap junctions, into the neighboring photocytes triggers a localized luminescence response. Following intense stimulation, the local rise in calcium elicits a secondary wave of luminescence that is supported by a voltage-independent calcium permeability mechanism in the photocyte plasma membrane. These two mechanisms for elevating internal calcium in light-emitting cells can account for the spatial and temporal features of intra-cellular luminescence in Obelia.",

author = "Paul Brehm and James Lechleiter and Stephen Smith and Kathleen Dunlap",

note = "Funding Information: The authors thank Drs. L. Jaffe and R. Kado for helpful and T. Turner for critical comments on the manuscript. provided by a grant from the National Science (8503159) and by a Grass Fellowship to 1. L.",

year = "1989",

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doi = "10.1016/0896-6273(89)90032-9",

language = "English (US)",

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pages = "191--198",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

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TY - JOUR

T1 - Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence

AU - Brehm, Paul

AU - Lechleiter, James

AU - Smith, Stephen

AU - Dunlap, Kathleen

N1 - Funding Information: The authors thank Drs. L. Jaffe and R. Kado for helpful and T. Turner for critical comments on the manuscript. provided by a grant from the National Science (8503159) and by a Grass Fellowship to 1. L.

PY - 1989/8

Y1 - 1989/8

N2 - Bioluminescence in the hydrozoan coelenterate Obelia results from calcium activation of a photoprotein contained in light-emitting cells (photocytes) scattered in the animal's endoderm. The influx of calcium into non-luminescent endodermal cells through conventional voltage-dependent calcium channels is required for the excitation-luminescence coupling. Our results suggest that the subsequent diffusion of this calcium, via gap junctions, into the neighboring photocytes triggers a localized luminescence response. Following intense stimulation, the local rise in calcium elicits a secondary wave of luminescence that is supported by a voltage-independent calcium permeability mechanism in the photocyte plasma membrane. These two mechanisms for elevating internal calcium in light-emitting cells can account for the spatial and temporal features of intra-cellular luminescence in Obelia.

AB - Bioluminescence in the hydrozoan coelenterate Obelia results from calcium activation of a photoprotein contained in light-emitting cells (photocytes) scattered in the animal's endoderm. The influx of calcium into non-luminescent endodermal cells through conventional voltage-dependent calcium channels is required for the excitation-luminescence coupling. Our results suggest that the subsequent diffusion of this calcium, via gap junctions, into the neighboring photocytes triggers a localized luminescence response. Following intense stimulation, the local rise in calcium elicits a secondary wave of luminescence that is supported by a voltage-independent calcium permeability mechanism in the photocyte plasma membrane. These two mechanisms for elevating internal calcium in light-emitting cells can account for the spatial and temporal features of intra-cellular luminescence in Obelia.

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JO - Neuron

JF - Neuron

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Intercellular signaling as visualized by endogenous calcium-dependent bioluminescence

Abstract

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