Interactions between human granulocytes and Blastomyces dermatitidis

J. W. Sixbey, B. T. Fields, C. N. Sun, Robert A Clark, C. M. Nolan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The authors studied interactions in vitro between human granulocytes and the yeast-like form of Blastomyces dermatitidis, because granulocytes are prominent in the host response to systemic blastomycosis. In Boyden chamber assays, broth culture filtrates of B. dermatitidis contained levels of granulocyte chemotactic activity that were significantly higher than those present in similar culture filtrates of Histoplasma capsulatum and Cryptococcus neoformans, two fungi that characteristically do not elicit granulocytes in infected tissues. Microscopic study, including electron microscopy, demonstrated that granulocytes phagocytosed B. dermatitidis promptly and efficiently. Moreover, granulocytes emitted light (chemiluminescence) at a brisk rate during phagocytosis of B. dermatitidis, indicating activation of intracellular metabolic pathways. However, fungicidal assays showed that granulocytes (1:1 cell-yeast ratio, 10% serum) killed only 29% of the B. dermatitidis inoculum during 3 h of incubation. Taken together, these findings suggest that there is disparity between phagocytosis and intracellular killing of B. dermatitidis by human granulocytes, perhaps because of resistance of this fungus to granulocyte microbicidal mechanisms.

Original languageEnglish (US)
Pages (from-to)41-44
Number of pages4
JournalInfection and Immunity
Volume23
Issue number1
StatePublished - 1979
Externally publishedYes

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Blastomyces
Granulocytes
Phagocytosis
Fungi
Yeasts
Blastomycosis
Histoplasma
Cryptococcus neoformans
Metabolic Networks and Pathways
Luminescence
Electron Microscopy

ASJC Scopus subject areas

  • Immunology

Cite this

Sixbey, J. W., Fields, B. T., Sun, C. N., Clark, R. A., & Nolan, C. M. (1979). Interactions between human granulocytes and Blastomyces dermatitidis. Infection and Immunity, 23(1), 41-44.

Interactions between human granulocytes and Blastomyces dermatitidis. / Sixbey, J. W.; Fields, B. T.; Sun, C. N.; Clark, Robert A; Nolan, C. M.

In: Infection and Immunity, Vol. 23, No. 1, 1979, p. 41-44.

Research output: Contribution to journalArticle

Sixbey, JW, Fields, BT, Sun, CN, Clark, RA & Nolan, CM 1979, 'Interactions between human granulocytes and Blastomyces dermatitidis', Infection and Immunity, vol. 23, no. 1, pp. 41-44.
Sixbey, J. W. ; Fields, B. T. ; Sun, C. N. ; Clark, Robert A ; Nolan, C. M. / Interactions between human granulocytes and Blastomyces dermatitidis. In: Infection and Immunity. 1979 ; Vol. 23, No. 1. pp. 41-44.
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