Interaction of virulent Mycoplasma pneumoniae with hamster tracheal organ cultures

P. C. Hu, A. M. Collier, J. B. Baseman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Exposure of hamster tracheal organ cultures to virulent Mycoplasma pneumoniae leads to alterations in ribonucleic acid (RNA) and protein biosynthesis and metabolism of the respiratory epithelium. An examination of the turnover rates of RNA and protein in infected tracheal organ cultures indicates that the rates of degradation of both prelabeled host cell RNA and protein are similar to those of uninfected controls. Infected tracheal organ cultures shifted to a nonpermissive medium within 24 h after infection and further incubated in the nonpermissive medium for 72 or 96 h behaved as normal uninfected cultures in terms of metabolic precursor uptake. Under these conditions, mycoplasmas remained attached to the respiratory epithelium. Cell membranes prepared from virulent mycoplasmas by several procedures neither attached to nor altered the metabolic activity of tracheal cultures. These data indicate that the intimate contact between virulent mycoplasmas and the respiratory epithelium does not alone account for the subsequent interruption of host cell metabolism but must be accompanied by continued multiplication and biochemical function of attached mycoplasmas.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalInfection and Immunity
Volume14
Issue number1
StatePublished - 1976
Externally publishedYes

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Mycoplasma pneumoniae
Mycoplasma
Organ Culture Techniques
Cricetinae
Respiratory Mucosa
RNA
Protein Biosynthesis
Proteins
Cell Membrane
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Hu, P. C., Collier, A. M., & Baseman, J. B. (1976). Interaction of virulent Mycoplasma pneumoniae with hamster tracheal organ cultures. Infection and Immunity, 14(1), 217-224.

Interaction of virulent Mycoplasma pneumoniae with hamster tracheal organ cultures. / Hu, P. C.; Collier, A. M.; Baseman, J. B.

In: Infection and Immunity, Vol. 14, No. 1, 1976, p. 217-224.

Research output: Contribution to journalArticle

Hu, PC, Collier, AM & Baseman, JB 1976, 'Interaction of virulent Mycoplasma pneumoniae with hamster tracheal organ cultures', Infection and Immunity, vol. 14, no. 1, pp. 217-224.
Hu, P. C. ; Collier, A. M. ; Baseman, J. B. / Interaction of virulent Mycoplasma pneumoniae with hamster tracheal organ cultures. In: Infection and Immunity. 1976 ; Vol. 14, No. 1. pp. 217-224.
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