Interaction of the N-Terminal Domain of the AMPA Receptor GluR4 Subunit with the Neuronal Pentraxin NP1 Mediates GluR4 Synaptic Recruitment

Gek-Ming Sia, Jean Claude Béïque, Gavin Rumbaugh, Richard Cho, Paul F. Worley, Richard L. Huganir

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Synaptogenesis requires recruitment of neurotransmitter receptors to developing postsynaptic specializations. We developed a coculture system reconstituting artificial synapses between neurons and nonneuronal cells to investigate the molecular components required for AMPA-receptor recruitment to synapses. With this system, we find that excitatory axons specifically express factors that recruit the AMPA receptor GluR4 subunit to sites of contact between axons and GluR4-transfected nonneuronal cells. Furthermore, the N-terminal domain (NTD) of GluR4 is necessary and sufficient for its recruitment to these artificial synapses and also for GluR4 recruitment to native synapses. Moreover, we show that axonally derived neuronal pentraxins NP1 and NPR are required for GluR4 recruitment to artificial and native synapses. RNAi knockdown and knockout of the neuronal pentraxins significantly decreases GluR4 targeting to synapses. Our results indicate that NP1 and NPR secreted from presynaptic neurons bind to the GluR4 NTD and are critical trans-synaptic factors for GluR4 recruitment to synapses.

Original languageEnglish (US)
Pages (from-to)87-102
Number of pages16
JournalNeuron
Volume55
Issue number1
DOIs
StatePublished - Jul 5 2007
Externally publishedYes

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Synapses
Axons
Neurons
Neurotransmitter Receptor
AMPA Receptors
Coculture Techniques
RNA Interference
AMPA 4 glutamate receptor ionotropic
neuronal pentraxin

Keywords

  • CELLBIO
  • MOLNEURO
  • SIGNALING

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Interaction of the N-Terminal Domain of the AMPA Receptor GluR4 Subunit with the Neuronal Pentraxin NP1 Mediates GluR4 Synaptic Recruitment. / Sia, Gek-Ming; Béïque, Jean Claude; Rumbaugh, Gavin; Cho, Richard; Worley, Paul F.; Huganir, Richard L.

In: Neuron, Vol. 55, No. 1, 05.07.2007, p. 87-102.

Research output: Contribution to journalArticle

Sia, Gek-Ming ; Béïque, Jean Claude ; Rumbaugh, Gavin ; Cho, Richard ; Worley, Paul F. ; Huganir, Richard L. / Interaction of the N-Terminal Domain of the AMPA Receptor GluR4 Subunit with the Neuronal Pentraxin NP1 Mediates GluR4 Synaptic Recruitment. In: Neuron. 2007 ; Vol. 55, No. 1. pp. 87-102.
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