TY - JOUR
T1 - Interaction of protein SRP19 with signal recognition particle RNA lacking individual RNA-helices
AU - Zwieb, Christian
PY - 1991/6/11
Y1 - 1991/6/11
N2 - Derivatives of human SRP-RNA were constructed by site-directed mutagenesis and tested for their ability to interact with protein SRP19. An RNA missing helix 6 barely interacts with SRP19, while the helix 8-deletion mutant retains much binding capability. A mutant RNA consisting just of helix 6 also binds the protein, but not as well as the unaltered molecule. SRP19 interacts to a full extent with the fourth mutant RNA composed of helices 6, 7, 8 and a portion of helix 5. It is concluded that helix 6-and not helix 8-is the major SRP19 binding site. Helices 7, 8 and portions of helix 5 contribute to the formation of a functional site. These results agree with data suggesting a proximity of helix 6 and the conserved part of SRP-RNA.
AB - Derivatives of human SRP-RNA were constructed by site-directed mutagenesis and tested for their ability to interact with protein SRP19. An RNA missing helix 6 barely interacts with SRP19, while the helix 8-deletion mutant retains much binding capability. A mutant RNA consisting just of helix 6 also binds the protein, but not as well as the unaltered molecule. SRP19 interacts to a full extent with the fourth mutant RNA composed of helices 6, 7, 8 and a portion of helix 5. It is concluded that helix 6-and not helix 8-is the major SRP19 binding site. Helices 7, 8 and portions of helix 5 contribute to the formation of a functional site. These results agree with data suggesting a proximity of helix 6 and the conserved part of SRP-RNA.
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U2 - 10.1093/nar/19.11.2955
DO - 10.1093/nar/19.11.2955
M3 - Article
C2 - 1711676
AN - SCOPUS:0025765510
SN - 0305-1048
VL - 19
SP - 2955
EP - 2960
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 11
ER -