Interaction of phomopsin A with normal and subtilisin-treated bovine brain tubulin

Asish Ray Chaudhuri, Richard F. Ludueña

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tubulin, the major component of microtubules, has a tendency to lose its ability to assemble or to bind to ligands in a time-dependent process known as "decay," The decay process also causes tubulin to expose sulfhydryl groups and hydrophobic areas. The antimitotic drug phomopsin A strongly protects the tubulin molecule from decay. Here we have studied the interaction of phomopsin A with αβ tubulin and tubulin which has been treated with subtilisin to remove selectively the C-termini of the α and βchains (αsβs). The binding of phomopsin A to αβ tubulin decreases the sulfhydryl titer by approximately 1.0 mol/mol. Selective removal of the peptides from the C-terminal ends does not affect phomopsin A's interaction with tubulin. Moreover, the αsβs tubulin-phomopsin A complex appears to be more stable than the αβ tubulin-phomopsin A complex as determined by the time-dependent increase in exposure of sulfhydryl groups and hydrophobic areas on tubulin. In fact, phomopsin A inhibits the decay process of αsβs tubulin completely. This observation raises the possibility of determining the conformtion of this configuration of tubulin.

Original languageEnglish (US)
Pages (from-to)99-105
Number of pages7
JournalProtein Journal
Volume16
Issue number2
StatePublished - 1997

Fingerprint

Subtilisin
Tubulin
Peptides
Brain
Ligands
Molecules
phomopsin
Antimitotic Agents
Microtubules

Keywords

  • Alkylation
  • C-terminal domain
  • Hydrophobic areas
  • Phomopsin A
  • Tubulin

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Organic Chemistry
  • Bioengineering

Cite this

Interaction of phomopsin A with normal and subtilisin-treated bovine brain tubulin. / Chaudhuri, Asish Ray; Ludueña, Richard F.

In: Protein Journal, Vol. 16, No. 2, 1997, p. 99-105.

Research output: Contribution to journalArticle

Chaudhuri, AR & Ludueña, RF 1997, 'Interaction of phomopsin A with normal and subtilisin-treated bovine brain tubulin', Protein Journal, vol. 16, no. 2, pp. 99-105.
Chaudhuri, Asish Ray ; Ludueña, Richard F. / Interaction of phomopsin A with normal and subtilisin-treated bovine brain tubulin. In: Protein Journal. 1997 ; Vol. 16, No. 2. pp. 99-105.
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