Interaction of benzo[a]pyrene diol-epoxide with nuclei and isolated chromatin

A. Kootstra, Thomas J Slaga, D. E. Olins

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Chicken erythrocyte chromatin and nuclei were labeled with benzo[a]pyrene (B[a]P) diol-epoxide (anti) and digested with micrococcal nuclease to mono- and dinucleosomes. Analysis of the distribution of the carcinogen showed that the internucleosomal region bound 3-4 times more carcinogen per unit DNA than did nucleosomes. The enhanced binding of the 'ultimate' carcinogen to the internucleosomal region was similar when isolated chromatin or nuclei were used for in vitro labeling. Furthermore, isolation of the histone core proteins, H2A, H2B, H3 and H4, revealed that only 15% of the carcinogen was associated with the histones and that the majority of the carcinogen was bound to chromosomal DNA. Fluorography of purified nucleosomal histones showed that the covalent association of the carcinogen was mainly with histones H3 and H2B.

Original languageEnglish (US)
Pages (from-to)225-236
Number of pages12
JournalChemico-Biological Interactions
Volume28
Issue number2-3
DOIs
StatePublished - 1979
Externally publishedYes

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Benzo(a)pyrene
Epoxy Compounds
Carcinogens
Chromatin
Histones
Nucleosomes
Photofluorography
Micrococcal Nuclease
DNA
Labeling
Chickens
Erythrocytes
Proteins

ASJC Scopus subject areas

  • Toxicology

Cite this

Interaction of benzo[a]pyrene diol-epoxide with nuclei and isolated chromatin. / Kootstra, A.; Slaga, Thomas J; Olins, D. E.

In: Chemico-Biological Interactions, Vol. 28, No. 2-3, 1979, p. 225-236.

Research output: Contribution to journalArticle

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