Integrative proteome analysis of Brachypodium distachyon roots and leaves reveals a synergetic responsive network under H2O2 stress

Yan Wei Bian, Dong Wen Lv, Zhi Wei Cheng, Ai Qin Gu, Hui Cao, Yue Ming Yan

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The plant oxidative stress response is vital for defense against various abiotic and biotic stresses. In this study, ultrastructural changes and the proteomic response to H2O2 stress in roots and leaves of the model plant Brachypodium distachyon were studied. Transmission electron microscopy (TEM) showed that the ultrastructural damage in roots was more serious than in leaves. Particularly, the ultrastructures of organelles and the nucleus in root tip cells were damaged, leading to the inhibition of normal biological activities of roots, which then spread throughout the plant. Based on two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF-MS, 84 and 53 differentially accumulated protein (DAP) spots representing 75 and 45 unique proteins responsive to H2O2 stress in roots and leaves, respectively, were identified. These protein species were mainly involved in signal transduction, energy metabolism, redox homeostasis/stress defense, protein folding/degradation, and cell wall/cell structure. Interestingly, two 14-3-3 proteins (GF14-B and GF14-D) were identified as DAPs in both roots and leaves. Protein-protein interaction (PPI) analysis revealed a synergetic H2O2-responsive network.

Original languageEnglish (US)
Pages (from-to)388-402
Number of pages15
JournalJournal of Proteomics
Volume128
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • 14-3-3 proteins
  • Brachypodium distachyon
  • HO stress
  • Leaf
  • Proteome
  • Root

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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