Integrative analysis reveals the transcriptional collaboration between EZH2 and E2F1 in the regulation of cancer-related gene expression

Han Xu, Kexin Xu, Housheng H. He, Chongzhi Zang, Chen Hao Chen, Yiwen Chen, Qian Qin, Su Wang, Chenfei Wang, Shengen Hu, Fugen Li, Henry Long, Myles Brown, X. Shirley Liu

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Overexpression of EZH2 is frequently linked to the advanced and metastatic stage of cancers. The mechanisms of its oncogenic function can be context specific, and may vary depending on the protein complexes that EZH2 interacts with. To identify novel transcriptional collaborators of EZH2 in cancers, a computational approach was developed that integrates protein- DNA binding data, cell perturbation gene expression data, and compendiums of tumor expression profiles. This holistic approach identified E2F1, a known mediator of the Rb tumor suppressor, as a transcriptional collaborator of EZH2 in castration- resistant prostate cancer. Subsequent analysis and experimental validation found EZH2 and E2F1 cobind to a subset of chromatin sites lacking H3K27 trimethylation, and activate genes that are critical for prostate cancer progression. The collaboration of EZH2 and E2F1 in transcriptional regulation is also observed in diffuse large B-cell lymphoma cell lines, where activation of the transcriptional network is concordant with the cellular response to the EZH2 inhibitor. Implications: The direct collaboration between EZH2 and Rb/E2F1 pathway provides an innovative mechanism underlying the cascade of tumor progression, and lays the foundation for the development of new anticancer targets/strategies.

Original languageEnglish (US)
Pages (from-to)163-172
Number of pages10
JournalMolecular Cancer Research
Volume14
Issue number2
DOIs
StatePublished - Feb 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Oncology
  • Cancer Research

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