Integration of synthetic globin genes into an E. coli plasmid

K. O. Wood, J. C. Lee

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Rabbit globin mRNA has been purified and used as a template by reverse transcriptase. The resulting duplex molecule consisting of rabbit globin mRNA/cDNA has been linked in vitro to Eco RI cleaved plasmid Col E1 DNA. Transformation of E. coli C600 by this recombinant molecule has been achieved. Transformed bacteria acquire the colicin E1 immunity of Col E1 and a closed circular DNA species of 4.40 - 4.45 x 106 daltons in molecular weight, an increase of 2.0 - 2.5 x 105 daltons compared to that of the parent plasmid DNA. In addition, 3H cDNA synthesized from globin RNA hybridizes preferentially to the recombinant plasmid DNA.

Original languageEnglish (US)
Pages (from-to)1961-1971
Number of pages11
JournalNucleic acids research
Volume3
Issue number8
DOIs
StatePublished - 1976

ASJC Scopus subject areas

  • Genetics

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