Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications

J. W. Parks, M. A. Olson, J. Kim, D. Ozcelik, H. Cai, R. Carrion, J. L. Patterson, R. A. Mathies, A. R. Hawkins, H. Schmidt

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    33 Scopus citations

    Abstract

    We describe the integration of an actively controlled programmable microfluidic sample processor with on-chip optical fluorescence detection to create a single, hybrid sensor system. An array of lifting gate microvalves (automaton) is fabricated with soft lithography, which is reconfigurably joined to a liquid-core, anti-resonant reflecting optical waveguide (ARROW) silicon chip fabricated with conventional microfabrication. In the automaton, various sample handling steps such as mixing, transporting, splitting, isolating, and storing are achieved rapidly and precisely to detect viral nucleic acid targets, while the optofluidic chip provides single particle detection sensitivity using integrated optics. Specifically, an assay for detection of viral nucleic acid targets is implemented. Labeled target nucleic acids are first captured and isolated on magnetic microbeads in the automaton, followed by optical detection of single beads on the ARROW chip. The combination of automated microfluidic sample preparation and highly sensitive optical detection opens possibilities for portable instruments for point-of-use analysis of minute, low concentration biological samples.

    Original languageEnglish (US)
    Article number054111
    JournalBiomicrofluidics
    Volume8
    Issue number5
    DOIs
    StatePublished - Sep 30 2014

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    ASJC Scopus subject areas

    • Biomedical Engineering
    • Materials Science(all)
    • Condensed Matter Physics
    • Fluid Flow and Transfer Processes
    • Colloid and Surface Chemistry

    Cite this

    Parks, J. W., Olson, M. A., Kim, J., Ozcelik, D., Cai, H., Carrion, R., Patterson, J. L., Mathies, R. A., Hawkins, A. R., & Schmidt, H. (2014). Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications. Biomicrofluidics, 8(5), [054111]. https://doi.org/10.1063/1.4897226