Integrating genomic analysis with the genetic basis of gene expression: Preliminary evidence of the identification of causal genes for cardiovascular and metabolic traits related to nutrition in Mexicans

Raúl A. Bastarrachea, Esther C. Gallegos-Cabriales, Edna J. Nava-González, Karin Haack, V. Saroja Voruganti, Jac Charlesworth, Hugo A. Laviada-Molina, Rosa A. Veloz-Garza, Velia Margarita Cardenas-Villarreal, Salvador B. Valdovinos-Chavez, Patricia Gomez-Aguilar, Guillermo Meléndez, Juan Carlos López-Alvarenga, Harald H.H. Göring, Shelley A. Cole, John Blangero, Anthony G. Comuzzie, Jack W. Kent

    Research output: Contribution to journalArticle

    6 Scopus citations

    Abstract

    Whole-transcriptome expression profiling provides novel phenotypes for analysis of complex traits. Gene expression measurements reflect quantitative variation in transcript-specific messenger RNA levels and represent phenotypes lying close to the action of genes. Understanding the genetic basis of gene expression will provide insight into the processes that connect genotype to clinically significant traits representing a central tenet of system biology. Synchronous in vivo expression profiles of lymphocytes, muscle, and subcutaneous fat were obtained from healthyMexican men. Most genes were expressed at detectable levels inmultiple tissues, and RNA levels were correlated between tissue types. A subset of transcripts with high reliability of expression across tissues (estimated by intraclass correlation coefficients) was enriched for cis-regulated genes, suggesting that proximal sequence variants may influence expression similarly in different cellular environments. This integrative global gene expression profiling approach is proving extremely useful for identifying genes and pathways that contribute to complex clinical traits. Clearly, the coincidence of clinical trait quantitative trait loci and expression quantitative trait loci can help in the prioritization of positional candidate genes. Such data will be crucial for the formal integration of positional and transcriptomic information characterized as genetical genomics.

    Original languageEnglish (US)
    Pages (from-to)596S-604S
    JournalAdvances in Nutrition
    Volume3
    Issue number4
    DOIs
    StatePublished - Jul 1 2012

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    ASJC Scopus subject areas

    • Food Science
    • Medicine (miscellaneous)
    • Nutrition and Dietetics

    Cite this

    Bastarrachea, R. A., Gallegos-Cabriales, E. C., Nava-González, E. J., Haack, K., Voruganti, V. S., Charlesworth, J., Laviada-Molina, H. A., Veloz-Garza, R. A., Cardenas-Villarreal, V. M., Valdovinos-Chavez, S. B., Gomez-Aguilar, P., Meléndez, G., López-Alvarenga, J. C., Göring, H. H. H., Cole, S. A., Blangero, J., Comuzzie, A. G., & Kent, J. W. (2012). Integrating genomic analysis with the genetic basis of gene expression: Preliminary evidence of the identification of causal genes for cardiovascular and metabolic traits related to nutrition in Mexicans. Advances in Nutrition, 3(4), 596S-604S. https://doi.org/10.3945/an.112.001925