Insulin resistance in uremia: An in vivo and in vitro study

Peter P. Stein, William A. Hunt, Carolyn M. Johnson, Ralph A. DeFronzo, J. Douglas Smith

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Insulin-mediated glucose metabolism was examined in vivo and in vitro in a chronically uremic (4-week) rat model established by a 90% nephrectomy. Using the euglycemic insulin clamp technique, uremic rats demonstrated a 28% reduction (P < .01) in total body glucose disposal compared with pair-fed controls. Suppression of hepatic glucose production by insulin was not impaired. The ability of insulin to promote glycogen synthesis by the soleus muscle in vitro was normal in uremic rats. In contrast, the ability of insulin to enhance both glycolysis and glucose oxidation by the soleus muscle was significantly reduced (P < .01) in uremic rats. These results provide evidence that at least two intracellular metabolic defects, ie, in the glycolytic and glucose oxidative pathways, contribute to the insulin resistance of chronic uremia.

Original languageEnglish (US)
Pages (from-to)562-567
Number of pages6
Issue number6
StatePublished - Jun 1989
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism


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