Insulin and IGF-I stimulate phosphorylation of their respective receptors in intact neuronal and glial cells in primary culture

Derek LeRoith, Joshua Shemer, Martin L Adamo, Mohan K. Raizada, Daphna Heffez, Yehiel Zick

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Previous studies have shown that insulin and IGF-I bind to their respective receptors and stimulate autophosphorylation of the receptor β subunits in detergent extracts of neuronal and glial cells. In the present study, intact neuronal and glial cells in primary culture have been utilized to characterize insulin- and IGF-I-stimulated phosphorylation of their receptors. Following [32P]orthophosphate labeling and stimulation by insulin or IGF-I, the cells were solubilized and the phosphorylated receptors were partially purified on wheat germ agglutinin-agarose columns, and immunoprecipitated using anti-phosphotyrosine or anti-insulin receptor antibodies. Insulin stimulated the phosphorylation of its receptor β subunit (95 kD phosphoprotein) in a dose-dependent manner, within at least 20 seconds in both neuronal and glial cells. Additionally, a 102-kD phosphoprotein was observed in insulin-stimulated neuronal cells. Maximal stimulation of receptor phosphorylation occurred at 1 minute for the glial cells, and 10 minutes for the neuronal cells. IGF-I stimulated the phosphorylation of two phosphoproteins in intact neuronal and glial cells; a 95-kD protein and a 102-kD protein, in a dose-dependent manner. These observations demonstrate that both insulin and IGF-I stimulate the phosphorylation of the β subunits of their respective receptors in brain cells in a similar fashion to their effects on receptors from nonneural tissues.

Original languageEnglish (US)
Pages (from-to)3-8
Number of pages6
JournalJournal of Molecular Neuroscience
Volume1
Issue number1
DOIs
StatePublished - Mar 1989
Externally publishedYes

Fingerprint

Phosphorylation
Insulin-Like Growth Factor I
Neuroglia
Insulin
Phosphoproteins
Insulin Antibodies
Wheat Germ Agglutinins
Phosphotyrosine
Insulin Receptor
Detergents
Sepharose
Labeling
Brain
Proteins
Phosphates
Tissue
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics
  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Insulin and IGF-I stimulate phosphorylation of their respective receptors in intact neuronal and glial cells in primary culture. / LeRoith, Derek; Shemer, Joshua; Adamo, Martin L; Raizada, Mohan K.; Heffez, Daphna; Zick, Yehiel.

In: Journal of Molecular Neuroscience, Vol. 1, No. 1, 03.1989, p. 3-8.

Research output: Contribution to journalArticle

LeRoith, Derek ; Shemer, Joshua ; Adamo, Martin L ; Raizada, Mohan K. ; Heffez, Daphna ; Zick, Yehiel. / Insulin and IGF-I stimulate phosphorylation of their respective receptors in intact neuronal and glial cells in primary culture. In: Journal of Molecular Neuroscience. 1989 ; Vol. 1, No. 1. pp. 3-8.
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