Insulin and cyclic AMP act at different levels on transcription of the L-type pyruvate kinase gene

Marie Hélène Cuif, Bruno Doiron, Axel Kahn

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We previously demonstrated that, in hepatocytes in primary culture, the role of insulin on induction of L-type pyruvate kinase (L-PK) gene expression was mainly to induce glucokinase synthesis, needed for glucose phosphorylation to glucose 6-phosphate. However, we show here that when hepatocytes have been isolated from rats starved for 72 h, glucose and constitutive glucokinase expression was not sufficient to fully stimulate the L-PR promoter, low insulin concentrations being still required. In addition, activation remains sensitive to cAMP inhibition, but cannot he reproduced in the absence of insulin by a competitive cAMP antagonist. We propose that both insulin and cAMP act on expression of the L-PK gene at, at least, two levels: positive and negative regulation of glucokinase gene expression, and more downstream levels.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalFEBS Letters
Volume417
Issue number1
DOIs
StatePublished - Nov 3 1997

Keywords

  • Cyclic AMP
  • Glucose
  • Insulin
  • L-type pyruvate kinase
  • Transcriptional control

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Insulin and cyclic AMP act at different levels on transcription of the L-type pyruvate kinase gene'. Together they form a unique fingerprint.

Cite this