Insights into the mechanisms of flavoprotein oxidases from kinetic isotope effects

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Deuterium, solvent, and 15N kinetic isotope effects have been used to probe the mechanisms by which flavoproteins oxidize carbon-oxygen and carbon-nitrogen bonds in amines, hydroxy acids, and alcohols. For the amine oxidases D-amino acid oxidase, N-methyltryptophan oxidase, and tryptophan monooxygenase, D-serine, sarcosine, and alanine are slow substrates for which CH bond cleavage is fully rate limiting. Inverse isotope effects for each of 0.992-0.996 are consistent with a common mechanism involving hydride transfer from the uncharged amine. Computational analyses of possible mechanisms support this conclusion. Deuterium and solvent isotope effects with wild-type and mutant variants of the lactate dehydrogenase flavocytochrome b2 show that OH and CH bond cleavage are not concerted, but become so in the Y254F enzyme. This is consistent with a highly asynchronous reaction in which OH bond cleavage precedes hydride transfer. The results of Hammett analyses and solvent and deuterium isotope effects support a similar mechanism for alcohol oxidase.

Original languageEnglish (US)
Pages (from-to)1016-1025
Number of pages10
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume50
Issue number11-12
DOIs
StatePublished - Oct 30 2007
Externally publishedYes

Fingerprint

Flavoproteins
Isotopes
Deuterium
Oxidoreductases
Amines
Kinetics
alcohol oxidase
Hydrides
L-Lactate Dehydrogenase (Cytochrome)
Carbon
D-Amino-Acid Oxidase
Sarcosine
Tryptophan Hydroxylase
Hydroxy Acids
L-Lactate Dehydrogenase
Alanine
Serine
Nitrogen
Alcohols
Oxygen

Keywords

  • Amine oxidase
  • Flavoprotein
  • Kinetic isotope effects
  • Mechanism

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Organic Chemistry
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology

Cite this

Insights into the mechanisms of flavoprotein oxidases from kinetic isotope effects. / Fitzpatrick, Paul F.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 50, No. 11-12, 30.10.2007, p. 1016-1025.

Research output: Contribution to journalArticle

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