Inorganic phosphate stimulates DMP1 expression in human periodontal ligament fibroblasts embedded in three-simensional collagen gels

Agnes D. Berendsen, Theo H. Smit, Ton Schoenmaker, X. Frank Walboomers, Stephen E Harris, Vincent Everts, Antonius L J J Bronckers

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Stable integration of collagenous tissue-engineered constructs to surrounding solid devices can be accomplished by coating the solid surfaces with exogenous alkaline phosphatase (ALP). We showed previously that coating of culture well surfaces with the enzyme in combination with the presence of its substrate β-glycerophosphate (β-GP) induces mineral deposition at the interface of matrix and surface, thereby preventing matrix detachment. In this study the effect of such mineral-inducing conditions on differentiation of human periodontal ligament (PDL) fibroblasts into osteoblasts/cementoblasts was analyzed in three-dimensional collagen gels. Mineral-inducing conditions decreased collagen type I gene expression and induced dentin matrix protein 1 (DMP1; a marker of late osteoblasts/cementoblasts) gene expression by fibroblasts. DMP1 protein was detected in some fibroblasts only in mineralizing gels. Exogenous ALP released high levels of inorganic phosphate from β-GP. Addition of inorganic phosphate alone induced DMP1 gene expression, which could be prevented by blocking phosphate entry into fibroblasts by foscarnet. We concluded that mineralizing conditions induced by exogenous ALP affect the phenotype of PDL fibroblasts. The fibroblasts are stimulated to express the late osteoblast/osteocyte marker protein DMP1, which is mediated by uptake of inorganic phosphate into the cells. The enzyme-mediated mineral deposition may thus facilitate enhanced integration of collagenous tissue-engineered constructs to devices or implants in vitro.

Original languageEnglish (US)
Pages (from-to)116-124
Number of pages9
JournalCells Tissues Organs
Volume192
Issue number2
DOIs
StatePublished - 2010

Fingerprint

Periodontal Ligament
Collagen
Fibroblasts
Gels
Phosphates
Minerals
Osteoblasts
Alkaline Phosphatase
Dental Cementum
Gene Expression
Foscarnet
Glycerophosphates
Osteocytes
Equipment and Supplies
Proteins
Dentin
Enzymes
Collagen Type I
Phenotype

Keywords

  • Alkaline phosphatase
  • Fibroblast
  • Hydroxyapatite
  • Osteoblast
  • Periodontium

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medicine(all)

Cite this

Berendsen, A. D., Smit, T. H., Schoenmaker, T., Walboomers, X. F., Harris, S. E., Everts, V., & Bronckers, A. L. J. J. (2010). Inorganic phosphate stimulates DMP1 expression in human periodontal ligament fibroblasts embedded in three-simensional collagen gels. Cells Tissues Organs, 192(2), 116-124. https://doi.org/10.1159/000289585

Inorganic phosphate stimulates DMP1 expression in human periodontal ligament fibroblasts embedded in three-simensional collagen gels. / Berendsen, Agnes D.; Smit, Theo H.; Schoenmaker, Ton; Walboomers, X. Frank; Harris, Stephen E; Everts, Vincent; Bronckers, Antonius L J J.

In: Cells Tissues Organs, Vol. 192, No. 2, 2010, p. 116-124.

Research output: Contribution to journalArticle

Berendsen, AD, Smit, TH, Schoenmaker, T, Walboomers, XF, Harris, SE, Everts, V & Bronckers, ALJJ 2010, 'Inorganic phosphate stimulates DMP1 expression in human periodontal ligament fibroblasts embedded in three-simensional collagen gels', Cells Tissues Organs, vol. 192, no. 2, pp. 116-124. https://doi.org/10.1159/000289585
Berendsen, Agnes D. ; Smit, Theo H. ; Schoenmaker, Ton ; Walboomers, X. Frank ; Harris, Stephen E ; Everts, Vincent ; Bronckers, Antonius L J J. / Inorganic phosphate stimulates DMP1 expression in human periodontal ligament fibroblasts embedded in three-simensional collagen gels. In: Cells Tissues Organs. 2010 ; Vol. 192, No. 2. pp. 116-124.
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