Initial characterization of the carbohydrate structure of MCP-1

Yanling Jiang; Lawrence A. Tabak; Anthony J. Valente; Dana T. Graves

doi:10.1016/0006-291X(91)91049-I

Initial characterization of the carbohydrate structure of MCP-1

Yanling Jiang, Lawrence A. Tabak, Anthony J. Valente, Dana T. Graves

Department of Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

MCP-1 is the principle monocyte chemoattractant produced by tumor cells in vitro. We previously identified different isoforms of MCP-1. Here we report that two predominant isoforms of MCP-1 (13 kD and 9 kD) can be distinguished by lectin binding. The results indicate that the disaccharide galactose-β1-3D-N-acetyl galactosamine is present on the 13 kD MCP-1 isoform but not the 9 kD isoform. Glycosylation of the 13 kD MCP-1 isoform does not appear to significantly change its antigenic properties and also does not affect its capacity to induce monocyte migration.

Original language	English (US)
Pages (from-to)	1400-1404
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	178
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)91049-I
State	Published - Aug 15 1991

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "MCP-1 is the principle monocyte chemoattractant produced by tumor cells in vitro. We previously identified different isoforms of MCP-1. Here we report that two predominant isoforms of MCP-1 (13 kD and 9 kD) can be distinguished by lectin binding. The results indicate that the disaccharide galactose-β1-3D-N-acetyl galactosamine is present on the 13 kD MCP-1 isoform but not the 9 kD isoform. Glycosylation of the 13 kD MCP-1 isoform does not appear to significantly change its antigenic properties and also does not affect its capacity to induce monocyte migration.",

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AB - MCP-1 is the principle monocyte chemoattractant produced by tumor cells in vitro. We previously identified different isoforms of MCP-1. Here we report that two predominant isoforms of MCP-1 (13 kD and 9 kD) can be distinguished by lectin binding. The results indicate that the disaccharide galactose-β1-3D-N-acetyl galactosamine is present on the 13 kD MCP-1 isoform but not the 9 kD isoform. Glycosylation of the 13 kD MCP-1 isoform does not appear to significantly change its antigenic properties and also does not affect its capacity to induce monocyte migration.

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Initial characterization of the carbohydrate structure of MCP-1

Abstract

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