Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells

A. J. Valente, S. R. Fowler, E. A. Sprague, J. L. Kelley, C. A. Suenram, C. J. Schwartz

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

A mononuclear cell chemoattractant of high specific activity produced by baboon (Papio cynocephalus) aortic medial smooth-muscle cells (SMCs) in culture has been partially characterized. Smooth-muscle cells, between the third and eighth passage, were grown to confluence in Medium 199 containing 10% fetal calf serum and then incubated for 24 hours in either serumless medium (Neuman and Tytell) or Medium 199 containing 0.2% bovine serum albumin. The 24-hour SMC-conditioned medium was fractionated on Sephadex G100-Superfine and potent chemoattractant activity (SMC-CF) eluted in the 10,000-12,000 dalton region. SMC-CF displayed chemotactic and chemokinetic activity for peripheral blood mononuclear cells but not for polymorphonuclear leukocytes. Production of SMC-CF by the cells was significantly inhibited in the presence of cycloheximide, and its activity was abolished after incubation with the bacterial protease subtilisin. Chromatofocusing experiments indicate that SMC-CF is a cationic protein with a pI of >10.5. The role SMC-CF may play as an inflammatory mediator in monocyte recruitment to the arterial intima in atherogenesis is discussed.

Original languageEnglish (US)
Pages (from-to)409-417
Number of pages9
JournalAmerican Journal of Pathology
Volume117
Issue number3
StatePublished - 1984

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells'. Together they form a unique fingerprint.

  • Cite this

    Valente, A. J., Fowler, S. R., Sprague, E. A., Kelley, J. L., Suenram, C. A., & Schwartz, C. J. (1984). Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells. American Journal of Pathology, 117(3), 409-417.