Inhibitory effect of melatonin on homocysteine-induced lipid peroxidation in rat brain homogenates

Carmen Osuna, Russel J Reiter, Joaquin J. García, Malgorzata Karbownik, Dun Xian Tan, Juan R. Calvo, Lucien C. Manchester

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Oxidative damage is implicated in several pathologies including cardiovascular disease. As a model system to study the response of cells to oxidative insults, homocysteine toxicity was examined since it is an independent risk factor for atherosclerotic disease. The levels of malondialdehyde and 4-hydroxyalkenals were assayed as an index of oxidatively damaged lipid. In in vitro experiments, the increase of lipid peroxidation products induced by homocysteine were concentration- and time-dependent. To study the protective effect of melatonin on homocystine induced lipid peroxidation, brain homogenates were treated with different concentrations of melatonin. The accumulation of malondialdehyde and 4-hydroxyalkenals induced by homocysteine was significantly reduced by melatonin in a concentration-dependent manner. Additionally, a melatonin concentration of 1.5 mM reduced the levels of oxidatively damaged lipid products below those measured in control homogenates (no homocysteine, no melatonin). These data suggest that melatonin, an endogenous antioxidant may have a role in protecting cells from oxidative damage due to homocysteine and they support the idea that pharmacological concentrations could be used as a therapeutic agent in reducing cardiovascular disease where homocysteine may be a causative or contributing agent.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalPharmacology and Toxicology
Volume90
Issue number1
DOIs
StatePublished - 2002

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Homocysteine
Melatonin
Lipid Peroxidation
Rats
Brain
Lipids
Malondialdehyde
Homocystine
Cardiovascular Diseases
Reducing Agents
Pathology
Toxicity
Antioxidants
Pharmacology
Experiments

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Inhibitory effect of melatonin on homocysteine-induced lipid peroxidation in rat brain homogenates. / Osuna, Carmen; Reiter, Russel J; García, Joaquin J.; Karbownik, Malgorzata; Tan, Dun Xian; Calvo, Juan R.; Manchester, Lucien C.

In: Pharmacology and Toxicology, Vol. 90, No. 1, 2002, p. 32-37.

Research output: Contribution to journalArticle

Osuna, Carmen ; Reiter, Russel J ; García, Joaquin J. ; Karbownik, Malgorzata ; Tan, Dun Xian ; Calvo, Juan R. ; Manchester, Lucien C. / Inhibitory effect of melatonin on homocysteine-induced lipid peroxidation in rat brain homogenates. In: Pharmacology and Toxicology. 2002 ; Vol. 90, No. 1. pp. 32-37.
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