Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness

Daniel R. Johnson, Jason O'connor, Robert Dantzer, Gregory G. Freund

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To the ill patient with diabetes, the behavioral symptoms of sickness such as fatigue and apathy are debilitating and can prevent recuperation. Here we report that peripherally administered insulin-like growth factor 1 (IGF-1) attenuates LPS-dependent depression of social exploration (sickness) in nondiabetic (db/+) but not in diabetic (db/db) mice. We show that the insulin IGF-1 mimetic vanadyl sulfate (VS) is effective at augmenting recovery from sickness in both db/+ and db/db mice. Specifically, peak illness was reached at 2 h for both VS and control animals injected with LPS, and VS mice recovered 50% faster than non-VS-treated animals. Examination of the mechanism of VS action in db/+ mice showed that VS paradoxically augmented peritoneal macrophage responsivity to LPS, increasing both peritoneal and ex vivo macrophage production of IL-1β and IL-6 but not TNF-α. The effects of VS in promoting recovery from sickness were not restricted to LPS, because they were also observed after direct administration of IL-1β. To explore the possibility that VS impairs immune-to-brain communication via vagal afferents, the vagally mediated satiety-inducing effects of cholecystokinin 8 were tested in db/+ mice. Cholecystokinin decreased food intake in saline-injected mice but not in VS-treated mice. VS also inhibited LPS-dependent up-regulation of IL-1β and IL-6 mRNA in the brain, while increasing by 50% the cerebral expression of transcripts of the specific antagonist of IL-1 receptors IL-IRA and IL-1R2. Taken together, these data indicate that VS improves recovery from LPS-induced sickness by blocking vagally mediated immune-to-brain signaling and by upregulating brain expression of IL-1β antagonists.

Original languageEnglish (US)
Pages (from-to)15184-15189
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number42
DOIs
StatePublished - Oct 18 2005
Externally publishedYes

Fingerprint

Brain
Interleukin-1
Somatomedins
Interleukin-6
vanadyl sulfate
Inhibition (Psychology)
Apathy
Behavioral Symptoms
Interleukin-1 Receptors
Cholecystokinin
Peritoneal Macrophages
Sulfates
Fatigue
Up-Regulation
Eating
Macrophages
Communication
Insulin
Messenger RNA

Keywords

  • Neuroimmunity
  • Sickness behavior
  • Type 2 diabetes
  • Vanadium

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness. / Johnson, Daniel R.; O'connor, Jason; Dantzer, Robert; Freund, Gregory G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 42, 18.10.2005, p. 15184-15189.

Research output: Contribution to journalArticle

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