Inhibition of the promotional phase of azoxymethane-induced colon carcinogenesis in the F344 rat by calcium lactate

effect of simulating two human nutrient density levels

Michael J Wargovich, D. Allnutt, C. Palmer, P. Anaya, L. C. Stephens

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The effects of 2 levels of dietary calcium and 2 types of dietary fat on the promotional phase phase of azoxymethane-induced colon cancer in the F344 rat were investigated. During the initiation phase of carcinogenesis all animals were fed a 5% corn oil AIN-76A diet containing 0.32% Ca in the form of calcium lactate. Rats were then injected with azoxymethane (AOM) weekly for 8 weeks. Thereafter, the rats were fed 1 of 3 diet formulations: a 5% corn oil diet or a 20% corn oil or 20% American Blend oil fat diet, with the level of Ca set at either 0.32% of the diet, a nutrient density simulating a daily human intake of ∼ 1700 mg Ca/day, or at 0.04% of the diet, reflecting a human daily intake of ∼ 200-250 mg of Ca/day, thus modeling 2 human nutrient density levels for calcium. Measurements of fecal pH during the experiment indicated an acidic adaptation of the large bowel to the lactate anion. Analysis of collected fecal samples showed more total fatty acids to be present in the colon when higher amounts of calcium were consumed. However, results of the tumorigenesis study indicated that calcium lactate fed at the 0.32% level significantly inhibited the development of colonic adenocarcinoma in all dietary groups. Taken together, this investigation supports the hypothesis that calcium supplementation can inhibit colon neoplasia in rats fed a high fat diet; however, under the conditions of this study, the 20% fat level did not significantly promote colon cancer as compared to a 5% fat level.

Original languageEnglish (US)
Pages (from-to)17-25
Number of pages9
JournalCancer Letters
Volume53
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Azoxymethane
Inbred F344 Rats
Colon
Carcinogenesis
Diet
Food
Corn Oil
Fats
Calcium
Colonic Neoplasms
Dietary Calcium
Dietary Fats
High Fat Diet
Anions
calcium lactate
Lactic Acid
Oils
Adenocarcinoma
Fatty Acids

Keywords

  • calcium
  • colon cancer
  • nutrient density

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Inhibition of the promotional phase of azoxymethane-induced colon carcinogenesis in the F344 rat by calcium lactate : effect of simulating two human nutrient density levels. / Wargovich, Michael J; Allnutt, D.; Palmer, C.; Anaya, P.; Stephens, L. C.

In: Cancer Letters, Vol. 53, No. 1, 1990, p. 17-25.

Research output: Contribution to journalArticle

@article{873475dc199c4313a85efff4b6d4797d,
title = "Inhibition of the promotional phase of azoxymethane-induced colon carcinogenesis in the F344 rat by calcium lactate: effect of simulating two human nutrient density levels",
abstract = "The effects of 2 levels of dietary calcium and 2 types of dietary fat on the promotional phase phase of azoxymethane-induced colon cancer in the F344 rat were investigated. During the initiation phase of carcinogenesis all animals were fed a 5{\%} corn oil AIN-76A diet containing 0.32{\%} Ca in the form of calcium lactate. Rats were then injected with azoxymethane (AOM) weekly for 8 weeks. Thereafter, the rats were fed 1 of 3 diet formulations: a 5{\%} corn oil diet or a 20{\%} corn oil or 20{\%} American Blend oil fat diet, with the level of Ca set at either 0.32{\%} of the diet, a nutrient density simulating a daily human intake of ∼ 1700 mg Ca/day, or at 0.04{\%} of the diet, reflecting a human daily intake of ∼ 200-250 mg of Ca/day, thus modeling 2 human nutrient density levels for calcium. Measurements of fecal pH during the experiment indicated an acidic adaptation of the large bowel to the lactate anion. Analysis of collected fecal samples showed more total fatty acids to be present in the colon when higher amounts of calcium were consumed. However, results of the tumorigenesis study indicated that calcium lactate fed at the 0.32{\%} level significantly inhibited the development of colonic adenocarcinoma in all dietary groups. Taken together, this investigation supports the hypothesis that calcium supplementation can inhibit colon neoplasia in rats fed a high fat diet; however, under the conditions of this study, the 20{\%} fat level did not significantly promote colon cancer as compared to a 5{\%} fat level.",
keywords = "calcium, colon cancer, nutrient density",
author = "Wargovich, {Michael J} and D. Allnutt and C. Palmer and P. Anaya and Stephens, {L. C.}",
year = "1990",
doi = "10.1016/0304-3835(90)90005-I",
language = "English (US)",
volume = "53",
pages = "17--25",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Inhibition of the promotional phase of azoxymethane-induced colon carcinogenesis in the F344 rat by calcium lactate

T2 - effect of simulating two human nutrient density levels

AU - Wargovich, Michael J

AU - Allnutt, D.

AU - Palmer, C.

AU - Anaya, P.

AU - Stephens, L. C.

PY - 1990

Y1 - 1990

N2 - The effects of 2 levels of dietary calcium and 2 types of dietary fat on the promotional phase phase of azoxymethane-induced colon cancer in the F344 rat were investigated. During the initiation phase of carcinogenesis all animals were fed a 5% corn oil AIN-76A diet containing 0.32% Ca in the form of calcium lactate. Rats were then injected with azoxymethane (AOM) weekly for 8 weeks. Thereafter, the rats were fed 1 of 3 diet formulations: a 5% corn oil diet or a 20% corn oil or 20% American Blend oil fat diet, with the level of Ca set at either 0.32% of the diet, a nutrient density simulating a daily human intake of ∼ 1700 mg Ca/day, or at 0.04% of the diet, reflecting a human daily intake of ∼ 200-250 mg of Ca/day, thus modeling 2 human nutrient density levels for calcium. Measurements of fecal pH during the experiment indicated an acidic adaptation of the large bowel to the lactate anion. Analysis of collected fecal samples showed more total fatty acids to be present in the colon when higher amounts of calcium were consumed. However, results of the tumorigenesis study indicated that calcium lactate fed at the 0.32% level significantly inhibited the development of colonic adenocarcinoma in all dietary groups. Taken together, this investigation supports the hypothesis that calcium supplementation can inhibit colon neoplasia in rats fed a high fat diet; however, under the conditions of this study, the 20% fat level did not significantly promote colon cancer as compared to a 5% fat level.

AB - The effects of 2 levels of dietary calcium and 2 types of dietary fat on the promotional phase phase of azoxymethane-induced colon cancer in the F344 rat were investigated. During the initiation phase of carcinogenesis all animals were fed a 5% corn oil AIN-76A diet containing 0.32% Ca in the form of calcium lactate. Rats were then injected with azoxymethane (AOM) weekly for 8 weeks. Thereafter, the rats were fed 1 of 3 diet formulations: a 5% corn oil diet or a 20% corn oil or 20% American Blend oil fat diet, with the level of Ca set at either 0.32% of the diet, a nutrient density simulating a daily human intake of ∼ 1700 mg Ca/day, or at 0.04% of the diet, reflecting a human daily intake of ∼ 200-250 mg of Ca/day, thus modeling 2 human nutrient density levels for calcium. Measurements of fecal pH during the experiment indicated an acidic adaptation of the large bowel to the lactate anion. Analysis of collected fecal samples showed more total fatty acids to be present in the colon when higher amounts of calcium were consumed. However, results of the tumorigenesis study indicated that calcium lactate fed at the 0.32% level significantly inhibited the development of colonic adenocarcinoma in all dietary groups. Taken together, this investigation supports the hypothesis that calcium supplementation can inhibit colon neoplasia in rats fed a high fat diet; however, under the conditions of this study, the 20% fat level did not significantly promote colon cancer as compared to a 5% fat level.

KW - calcium

KW - colon cancer

KW - nutrient density

UR - http://www.scopus.com/inward/record.url?scp=0025090489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025090489&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(90)90005-I

DO - 10.1016/0304-3835(90)90005-I

M3 - Article

VL - 53

SP - 17

EP - 25

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -