Inhibition of the glucocorticoid receptor expression by diverse tumor promoters in SENCAR mouse epidermis

Ho Il Kang, Irina V. Budunova, Steve Carbajal, Thomas J. Slaga

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Glucocorticoid hormones are very potent inhibitors of keratinocyte proliferation. Their function is mediated by the glucocorticoid receptor (GR) which is highly expressed in mouse epidermis. In the study reported here we compared the effect of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and non-phorbol ester tumor promoters such as okadaic acid, chrysarobin, and benzoyl peroxide on the levels of GR protein and mRNA in SENCAR mouse epidermis. Glucocorticoid binding assay and Northern blot analysis revealed that all four tumor promoters decreased both GR protein and mRNA levels in keratinocytes in vivo. We also found that TPA and okadaic acid inhibited GR expression in keratinocyte cell line. These results suggest that GR inhibition may play an important role in mouse skin hyperplasia and promotion of skin carcinogenesis.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalInternational journal of oncology
Volume10
Issue number2
StatePublished - Feb 1 1997
Externally publishedYes

Keywords

  • glucocorticoid receptor
  • mouse epidermis
  • tumor promoter

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Inhibition of the glucocorticoid receptor expression by diverse tumor promoters in SENCAR mouse epidermis'. Together they form a unique fingerprint.

  • Cite this