Inhibition of parathyroid hormone-responsive adenylate cyclase in clonal osteoblast-like cells by transforming growth factor α and epidermal growth factor

G. E. Gutierrez, G. R. Mundy, R. Derynck, E. L. Hewlett, M. S. Katz

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The effects of transforming growth factor α (TGF-α) and epidermal growth factor (EGF) on parathyroid hormone (PTH)-responsive adenylate cyclase were examined in clonal rat osteosarcoma cells (UMR-106) with the osteoblast phenotype. Recombinant TFG-α and EGF incubated with UMR-106 cells for 48 h each produced concentration-dependent inhibition of PTH-responsive adenylate cyclase, with maximal inhibition of 38-44% at 1-3 ng/ml of either growth factor. TGF-α and EGF also inhibited β-adrenergic agonist (isoproterenol)-stimulated adenylate cyclase by 32%, but neither growth factor affected enzyme response to prostaglandin or basal (unstimulated) activity. Nonreceptor-mediated activation of adenylate cyclase by forskolin and cholera toxin was inhibited 18-20% by TGF-α and EGF. Pertussis toxin augmented PTH-stimulated adenylate cyclase, suggesting modulation of PTH response by a functional inhibitory guanine nucleotide-binding regulatory component of the enzyme. However, pertussis toxin had no effect on TGF-α inhibition of PTH response. Growth factor inhibition of PTH response was time-dependent, with maximal inhibition by 4-12 h of TGF-α exposure, and was reduced by prior treatment of UMR-106 cells with cycloheximide. TGF-α was not mitogenic for UMR-106 cells. The results indicate that TGF-α and EGF selectively impair PTH- and β-adrenergic agonist-responsive adenylate cyclase of osteoblast-like cells. Growth factor inhibition of adenylate cyclase may be exerted at the receptor for stimulatory agonist and at nonreceptor components excluding pertussis toxin-sensitive guanine nucleotide-binding regulatory proteins. The inhibitory action of growth factors may also require protein synthesis. Inhibition of PTH responsiveness of osteoblasts by growth factors conceivably explains why solid tumors secreting growth factors in addition to factors which interact with PTH receptors may be associated with decreased bone formation.

Original languageEnglish (US)
Pages (from-to)15845-15850
Number of pages6
JournalJournal of Biological Chemistry
Volume262
Issue number33
StatePublished - 1987

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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