TY - JOUR
T1 - Inhibition of mitochondrial oxidative phosphorylation by 2-methyl-4-dimethylaminoazobenzene
AU - Kumar, P. Sai
AU - Kurup, C. K.Ramakrishna
N1 - Funding Information:
Financial assistance from the University Grants Commission, New Delhi is acknowledged.
PY - 1984/7/27
Y1 - 1984/7/27
N2 - The azodye 2-methyl-4-dimethylaminoazobenzene inhibited oxidation and phosphorylation in tightly coupled rat liver mitochondria. Phosphorylation was more sensitive to the inhibitory action of the azodye than was the oxidation of succinate or ascorbate. The oxidation of NAD+-linked substrate was severely inhibited by the compound. In submitochondrial particles, only NADH oxidation was sensitive. The site of inhibition has been identified to lie between the dehydrogenase flavoprotein and ubiquinone.
AB - The azodye 2-methyl-4-dimethylaminoazobenzene inhibited oxidation and phosphorylation in tightly coupled rat liver mitochondria. Phosphorylation was more sensitive to the inhibitory action of the azodye than was the oxidation of succinate or ascorbate. The oxidation of NAD+-linked substrate was severely inhibited by the compound. In submitochondrial particles, only NADH oxidation was sensitive. The site of inhibition has been identified to lie between the dehydrogenase flavoprotein and ubiquinone.
KW - (Rat liver mitochondria)
KW - 2-Methyl-4-dimethylaminoazobenzene
KW - Azodye
KW - Electron transport inhibitor
KW - Oxidative phosphorylation
KW - Respiratory chain
UR - http://www.scopus.com/inward/record.url?scp=0021217659&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021217659&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(84)90240-8
DO - 10.1016/0005-2728(84)90240-8
M3 - Article
C2 - 6430342
AN - SCOPUS:0021217659
VL - 766
SP - 263
EP - 266
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 1
ER -