Inhibition of intermediary metabolism by amiodarone in dog thyroid slices

D. Pasquali, F. Y. Tseng, Sheela R Kadapakkam, J. B. Field

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Amiodarone, an iodine-containing antiarrhythmic drug, has been reported to interfere with thyroid function and thyroid hormone metabolism. We studied the effects of amiodarone on basal and agonist [thyroid-stimulating hormone (TSH), phorbol ester, or carbachol]-stimulated glucose oxidation, 32PO4 incorporation into phospholipids, and adenosine 3',5'-cyclic monophosphate (cAMP) concentration in dog thyroid slices. Slices were preincubated with amiodarone at 37°C for 1 h before the addition of agonist and the appropriate radioisotope. cAMP stimulation was measured after 20 min, glucose oxidation for 45 min, and 32PO4 incorporation into phospholipids for 2 h. Amiodarone (0.5 mM) had no effect on basal 14CO2 formation or 32PO4 incorporation into phospholipids but significantly inhibited TSH, phorbol ester, and carbachol stimulation of these parameters. It also inhibited cAMP stimulation by TSH. Inhibition of TSH-stimulated [14C]glucose oxidation was also obtained with another iodide-containing compound, iopanoic acid (0.5 mM), but not with iothalamate (up to 10 mM). Inhibition by amiodarone was still present, but to a lesser extent, when it was added at the same time as the agonist. Inhibition of stimulated [14C]glucose oxidation persisted even after the slices were incubated without amiodarone for 6 h. Inhibition by amiodarone, in contrast to that by inorganic iodide, was not prevented by 1 mM methimazole added at the same time as amiodarone. These results indicate that the inhibitory effects of amiodarone on thyroid function are not due to dissociation of iodide from the molecule.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume259
Issue number4 22-4
StatePublished - 1990
Externally publishedYes

Fingerprint

Enzyme inhibition
Amiodarone
Metabolism
Thyroid Gland
Dogs
Thyrotropin
Iodides
Glucose
Phospholipids
Oxidation
Carbachol
Phorbol Esters
Iopanoic Acid
Iothalamic Acid
Methimazole
Anti-Arrhythmia Agents
Thyroid Hormones
Radioisotopes
Iodine
Cyclic AMP

Keywords

  • carbachol
  • iodide-containing compounds
  • methimazole
  • phorbol ester
  • thyroid-stimulating hormone

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Inhibition of intermediary metabolism by amiodarone in dog thyroid slices. / Pasquali, D.; Tseng, F. Y.; Kadapakkam, Sheela R; Field, J. B.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 259, No. 4 22-4, 1990.

Research output: Contribution to journalArticle

Pasquali, D. ; Tseng, F. Y. ; Kadapakkam, Sheela R ; Field, J. B. / Inhibition of intermediary metabolism by amiodarone in dog thyroid slices. In: American Journal of Physiology - Endocrinology and Metabolism. 1990 ; Vol. 259, No. 4 22-4.
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