Inhibition of H2O2-induced apoptosis of lymphocytes by calorie restriction during aging

C. P.Reddy Avula, Gabriel Fernandes

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Calorie restriction (CR) is known to delay the aging process in rodents and is postulated to act by decreasing free radical generation and increasing antioxidant enzyme activity. The present study was designed to investigate the effect of CR and age on oxidative stress-induced apoptosis and associated changes in the levels of TNF-α, and Bcl-2 in splenic T lymphocytes. Ad libitum (AL)- or CR-fed C57BL/6J mice were sacrificed either at 6 (young) or 18 (old) months and splenic lymphocytes were incubated with or without 25 μM H2O2 to induce apoptosis. Apoptosis increased with age in cells of AL-fed mice incubated with H2O2. CR prevented this rise in apoptosis in total splenic lymphocytes and in CD4+ and CD8+ T lymphocyte subsets either with or without H2O2. Free radicals increased and mitochondrial membrane potential decreased in aged mice. CR prevented these changes and also prevented the age-associated increase in TNF-α and loss of Bcl-2 in total splenic lymphocytes and in CD4+ and CD8+ lymphocyte subsets. In summary, lymphocytes in aged AL-fed mice were much more susceptible to oxidative stress-induced apoptosis whereas CR normalized apoptosis by preventing the increase in TNF-α and the decrease in Bcl-2 associated with aging.

Original languageEnglish (US)
Pages (from-to)282-292
Number of pages11
JournalMicroscopy Research and Technique
Issue number4
StatePublished - Nov 15 2002


  • Antioxidants
  • Apoptosis
  • Calorie restriction
  • Reactive oxygen species
  • TNF-αBcl-2

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology


Dive into the research topics of 'Inhibition of H<sub>2</sub>O<sub>2</sub>-induced apoptosis of lymphocytes by calorie restriction during aging'. Together they form a unique fingerprint.

Cite this