Inhibition of cerebellar nitric oxide synthase and cyclic GMP production by melatonin via complex formation with calmodulin

David Pozo, Russel J Reiter, Juan R. Calvo, Juan M. Guerrero

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

Constitutive rat cerebellar nitric oxide synthase (NOS) activity is shown to be inhibited by physiological concentrations of the pineal hormone melatonin. The inhibition was dose dependent and was coupled to an inhibition of the cyclic GMP production activated by L-arginine. Results also show that calmodulin appears to be involved in this process because its presence in the incubation medium was able to prevent the effect of melatonin on both NOS activity and cyclic GMP production. Moreover, polyacrylamide gel electrophoresis studies suggest that melatonin can interact with calmodulin modifying the binding of the peptide to the synthetic NOS peptide encompassing the calmodulinbinding domain of constitutive NOS from rat cerebellum, the natural mechanism by which calmodulin activates cerebellar NOS.

Original languageEnglish (US)
Pages (from-to)430-442
Number of pages13
JournalJournal of Cellular Biochemistry
Volume65
Issue number3
DOIs
StatePublished - Jun 1 1997

Fingerprint

Cyclic GMP
Melatonin
Calmodulin
Nitric Oxide Synthase
Rats
Peptides
Electrophoresis
Cerebellum
Arginine
Polyacrylamide Gel Electrophoresis
Hormones

Keywords

  • calmodulin
  • cerebellum
  • melatonin
  • nitric oxide
  • nitric oxide synthase
  • pineal gland

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Inhibition of cerebellar nitric oxide synthase and cyclic GMP production by melatonin via complex formation with calmodulin. / Pozo, David; Reiter, Russel J; Calvo, Juan R.; Guerrero, Juan M.

In: Journal of Cellular Biochemistry, Vol. 65, No. 3, 01.06.1997, p. 430-442.

Research output: Contribution to journalArticle

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