Inhibition of cellular activation of retroviral replication by CD8+ T cells derived from non-human primates

J. D. Powell, D. P. Bednarik, T. M. Folks, T. Jehuda-Cohen, F. Villinger, K. W. Sell, A. A. Ansari

    Research output: Contribution to journalArticle

    42 Scopus citations

    Abstract

    To test the hypothesis that CD8+ T cells inhibit viral replication at the level of cellular activation, an Epstein-Barr virus (EBV)-transformed cell line (FEc1) from a simian immunodeficiency virus (SIV)-seropositive sooty mangabey monkey was transfected with a human CD4 gene and shown to be replication-competent for HIV-1, HIV-2 and SIV. Utilizing a dual-chamber culture system, it was found that inhibition of viral replication can be mediated by a soluble factor. The FEc1 cell line was transiently transfected with an LTR-driven CAT reporter gene. It was found that autologous CD8+ T cells markedly inhibited CAT activity. Furthermore, co-transfection of the FEc1 cell line with an LTR-driven tat plasmid and LTR-CAT was able to quantitatively mitigate the suppressive effect. Thus, this inhibition appears to be directed at cellular mechanisms of viral transcription. Control transfections with an LTR-driven CAT plasmid with a mutation at the NFkB binding site yielded no CAT activity, suggesting that most viral replication as measured by CAT activity is dependent, to a large extent, upon cellularly derived NFkB binding proteins.

    Original languageEnglish (US)
    Pages (from-to)473-481
    Number of pages9
    JournalClinical and Experimental Immunology
    Volume91
    Issue number3
    DOIs
    StatePublished - 1993

    Keywords

    • AIDS
    • HIV
    • SIV
    • immunoregulation
    • virus suppression

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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