Inhibition of CD95-mediated apoptosis through β1 integrin in the HSG epithelial cell line

Howard Dang, Parastou Lizeth Dehghan, Kai Goodwiler, Shuo Chen, Gustavo Zardeneta, Bin Xian Zhang, Chih Ko Yeh

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The HSG cell line serves as a model for salivary gland epithelial progenitor cell differentiation. In order for a progenitor cell to differentiate, the cell must maintain viability within its niche. Studies were designed to elucidate the mechanism for integrin-mediated HSG cell survival. HSG cells, grown on Matrigel®, were resistant to CD95-mediated apoptosis. Western blot analysis showed that Matrigel® induced the expression of bcl-2, bcl-xL, p63, and ΔNp63. This induction occurred by as early as 2 hrs and remained for 24 hrs. CD95-mediated apoptosis resistance was dependent, however, upon the expression of the bcl-2 family. Furthermore, Matrigel® induced bcl-2 family expression was dependent on the transactivation of the EGF receptor pathway since PD98059 and AG1478 inhibited Matrigel® induced bcl-2 family expression and caused HSG cells to be sensitive to CD95-mediated apoptosis. Activation of the EGF receptor pathway, by itself, however, was not sufficient to inhibit apoptosis. Blocking antibody showed that bcl-2 family expression was mediated through β1 integrin. These studies show that salivary progenitor epithelial cell survival is integrin dependent and involves the transactivation of the EGF receptor pathway.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalCell Communication and Adhesion
Volume13
Issue number4
DOIs
Publication statusPublished - Jul 1 2006

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Keywords

  • Apoptosis
  • Bcl-2
  • CD95
  • Integrins
  • Salivary gland
  • p63

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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