Inhibition of bacteriophage M13 and phiX174 duplex DNA replication and single strand synthesis in temperature sensitive dnaZ mutants of Escherichia coli

W. G. Haldenwang, J. R. Walker

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A functional dnaZ product, known to be essential for host DNA polymerization and for the synthesis of M13 and phiX174 parental replicative-form (RF) DNA, is required also for RF replication and single-strand synthesis by both of these phages. All three stages of M13 and phiX174 DNA replication (parental RF formation, RF replication, and single-strand synthesis) are inhibited in dnaZts mutants at elevated temperatures. In addition, the thermolabile step in M13 parental RF formation appears to occur after RNA priming; i.e., the synthesis of M13 RF DNA proceeded when a dnaZts mutant, infected at a nonpermissive temperature, was transferred to a permissive temperature in the presence of rifampin.

Original languageEnglish (US)
Pages (from-to)23-30
Number of pages8
JournalJournal of virology
Volume22
Issue number1
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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