Influenza RNA not detected in archival brain tissues from acute encephalitis lethargica cases or in postencephalitic parkinson cases

Sherman Mccall, James M Henry, Ann H. Reid, Jeffery K. Taubenberger

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Encephalitis lethargica (EL) was a mysterious epidemic, temporally associated with the 1918 Spanish influenza pandemic. Numerous symptoms characterized this disease, including headache, diplopia, fever, fatal coma, delirium, oculogyric crisis, lethargy, catatonia, and psychiatric symptoms. Many patients who initially recovered subsequently developed profound, chronic parkinsonism. The etiologic association of influenza with EL is controversial. Five acute EL autopsies and more than 70 postencephalitic parkinsonian autopsies were available in the Armed Forces Institute of Pathology (AFIP) tissue repository. Two of these 5 acute EL cases had histopathologic changes consistent with that diagnosis. The remaining 3 cases were classified as possible acute EL cases as the autopsy material was insufficient for detailed histopathologic examination. RNA lysates were prepared from 29 CNS autopsy tissue blocks from the 5 acute cases and 9 lysates from blocks containing substantia nigra from 2 postencephalitic cases. RNA recovery was assessed by amplification of beta-2-microglobulin mRNA and 65% of the tissue blocks contained amplifiable RNA. Reverse transcription-polymerase chain reaction (RT-PCR) for influenza matrix and nucleoprotein genes was negative in all cases. Thus, it is unlikely that the 1918 influenza virus was neurotropic and directly responsible for the outbreak of EL.

Original languageEnglish (US)
Pages (from-to)696-704
Number of pages9
JournalJournal of Neuropathology and Experimental Neurology
Volume60
Issue number7
StatePublished - 2001
Externally publishedYes

Fingerprint

Encephalitis
Human Influenza
RNA
Autopsy
Brain
Catatonia
beta 2-Microglobulin
Lethargy
Nucleoproteins
Diplopia
Delirium
Parkinsonian Disorders
Pandemics
Substantia Nigra
Coma
Orthomyxoviridae
Reverse Transcription
Disease Outbreaks
Headache
Psychiatry

Keywords

  • Encephalitis lethargica
  • Influenza
  • Postencephalitic parkinsonism

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Influenza RNA not detected in archival brain tissues from acute encephalitis lethargica cases or in postencephalitic parkinson cases. / Mccall, Sherman; Henry, James M; Reid, Ann H.; Taubenberger, Jeffery K.

In: Journal of Neuropathology and Experimental Neurology, Vol. 60, No. 7, 2001, p. 696-704.

Research output: Contribution to journalArticle

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