Influence of topography on endothelialization of stents: Clues for new designs

Cristina Simon, Julio C. Palmaz, Eugene A. Sprague

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

To evaluate the influence of stent design on endothelialization of the stented surface, we placed trapezoidal objects of variable thickness on a confluent culture of endothelial cells and subjected the assembly to flow and shear conditions similar to those found in arteries. After 24 h, we measured and analyzed the area on top of the objects covered by cells and the maximum migration distance from the borders. In addition, we evaluated areas devoid of cells surrounding the objects, which developed after exposure of the assembly to flow. The cell-covered area and migration distance significantly decreased on objects 75 μm thick, and it was nonexistent on objects 250 μm thick. Areas devoid of cells or gaps were largest adjacent to the downflow side of the object, disposed transversely to flow. Cell gaps were smallest along the side aligned with flow. In conclusion, endothelial cell coverage may be impaired by stent wall thickness larger than 75 μm. It is likely that this impairment is related to flow disturbances impairing cell attachment.

Original languageEnglish (US)
Pages (from-to)143-151
Number of pages9
JournalJournal of Long-Term Effects of Medical Implants
Volume10
Issue number1-2
DOIs
StatePublished - Jan 1 2000

Keywords

  • Endothelium
  • Flow disturbances
  • Stents

ASJC Scopus subject areas

  • Biomedical Engineering
  • Dentistry(all)

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