Abstract
Adoptive secondary anti-acetylcholine receptor (AChR) antibody responses were examined in rats to evaluate the influence of helper T cell specificity on the nature and disease-causing potential of antibody produced. Mixtures of B cells reactive with the intact AChR plus T cells reactive with purified AChR subunits (α, β, γ, δ) were transferred and antigen-challenged in immunologically naive recipient rats; the serum anti-AChR antibody produced was assessed by radioimmunoassay for differences in titers and by isoelectric focusing for differences in clonal heterogeneity as a function of the subunit specificity of T cells transferred. In addition, rats receiving different sources of AChR or AChR subunit-reactive T cells were examined for AChR-dependent muscle dysfunction. The results indicated a clear reduction in anti-AChR antibody concentrations and clonal heterogeneity in recipient rats receiving T cells of specificities restricted to individual subunits. However, except for a clear relationship between serum anti-AChR antibody concentration and disease induction, no particular AChR subunit-reactive helper T cell specificity appeared to preferentially cause muscle dysfunction. We conclude that if such relationships exists, T cells with specificities more restricted than those described here will have to be used.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-34 |
| Number of pages | 18 |
| Journal | Journal of Neuroimmunology |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1987 |
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Keywords
- Acetylcholine receptor
- Autoantibody
- Muscle function
- Myasthenia gravis
- T cell specificity
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Clinical Neurology
- Neurology
Cite this
Influence of T cell specificity on the heterogeneity and disease-causing capability of antibody against the acetylcholine receptor. / Yeh, Trai Ming; Krolick, Keith A.
In: Journal of Neuroimmunology, Vol. 17, No. 1, 1987, p. 17-34.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of T cell specificity on the heterogeneity and disease-causing capability of antibody against the acetylcholine receptor
AU - Yeh, Trai Ming
AU - Krolick, Keith A
PY - 1987
Y1 - 1987
N2 - Adoptive secondary anti-acetylcholine receptor (AChR) antibody responses were examined in rats to evaluate the influence of helper T cell specificity on the nature and disease-causing potential of antibody produced. Mixtures of B cells reactive with the intact AChR plus T cells reactive with purified AChR subunits (α, β, γ, δ) were transferred and antigen-challenged in immunologically naive recipient rats; the serum anti-AChR antibody produced was assessed by radioimmunoassay for differences in titers and by isoelectric focusing for differences in clonal heterogeneity as a function of the subunit specificity of T cells transferred. In addition, rats receiving different sources of AChR or AChR subunit-reactive T cells were examined for AChR-dependent muscle dysfunction. The results indicated a clear reduction in anti-AChR antibody concentrations and clonal heterogeneity in recipient rats receiving T cells of specificities restricted to individual subunits. However, except for a clear relationship between serum anti-AChR antibody concentration and disease induction, no particular AChR subunit-reactive helper T cell specificity appeared to preferentially cause muscle dysfunction. We conclude that if such relationships exists, T cells with specificities more restricted than those described here will have to be used.
AB - Adoptive secondary anti-acetylcholine receptor (AChR) antibody responses were examined in rats to evaluate the influence of helper T cell specificity on the nature and disease-causing potential of antibody produced. Mixtures of B cells reactive with the intact AChR plus T cells reactive with purified AChR subunits (α, β, γ, δ) were transferred and antigen-challenged in immunologically naive recipient rats; the serum anti-AChR antibody produced was assessed by radioimmunoassay for differences in titers and by isoelectric focusing for differences in clonal heterogeneity as a function of the subunit specificity of T cells transferred. In addition, rats receiving different sources of AChR or AChR subunit-reactive T cells were examined for AChR-dependent muscle dysfunction. The results indicated a clear reduction in anti-AChR antibody concentrations and clonal heterogeneity in recipient rats receiving T cells of specificities restricted to individual subunits. However, except for a clear relationship between serum anti-AChR antibody concentration and disease induction, no particular AChR subunit-reactive helper T cell specificity appeared to preferentially cause muscle dysfunction. We conclude that if such relationships exists, T cells with specificities more restricted than those described here will have to be used.
KW - Acetylcholine receptor
KW - Autoantibody
KW - Muscle function
KW - Myasthenia gravis
KW - T cell specificity
UR - http://www.scopus.com/inward/record.url?scp=0011844367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0011844367&partnerID=8YFLogxK
U2 - 10.1016/0165-5728(87)90028-2
DO - 10.1016/0165-5728(87)90028-2
M3 - Article
C2 - 2960692
AN - SCOPUS:0011844367
VL - 17
SP - 17
EP - 34
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1
ER -