TY - JOUR
T1 - Influence of T cell specificity on the antibody response to the acetylcholine receptor
AU - Krolick, Keith A.
AU - Urso, Olgui E.
N1 - Funding Information:
This work was supported by a grant from the Carneiro, Chumney and Co. Morrison Trust Fund and the National Institutes of Health Grant NS 8938.
PY - 1986/11
Y1 - 1986/11
N2 - Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.
AB - Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.
KW - Acetylcholine receptor
KW - Autoimmunity
KW - Myasthenia gravis
KW - T cells
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U2 - 10.1016/0165-5728(86)90051-2
DO - 10.1016/0165-5728(86)90051-2
M3 - Article
C2 - 2428835
AN - SCOPUS:0022486820
VL - 13
SP - 75
EP - 87
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1
ER -