Abstract
Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 75-87 |
| Number of pages | 13 |
| Journal | Journal of Neuroimmunology |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1986 |
Fingerprint
Keywords
- Acetylcholine receptor
- Autoimmunity
- Myasthenia gravis
- T cells
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Clinical Neurology
- Neurology
Cite this
Influence of T cell specificity on the antibody response to the acetylcholine receptor. / Krolick, Keith A; Urso, Olgui E.
In: Journal of Neuroimmunology, Vol. 13, No. 1, 1986, p. 75-87.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of T cell specificity on the antibody response to the acetylcholine receptor
AU - Krolick, Keith A
AU - Urso, Olgui E.
PY - 1986
Y1 - 1986
N2 - Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.
AB - Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.
KW - Acetylcholine receptor
KW - Autoimmunity
KW - Myasthenia gravis
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=0022486820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022486820&partnerID=8YFLogxK
U2 - 10.1016/0165-5728(86)90051-2
DO - 10.1016/0165-5728(86)90051-2
M3 - Article
C2 - 2428835
AN - SCOPUS:0022486820
VL - 13
SP - 75
EP - 87
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1
ER -