Influence of T cell specificity on the antibody response to the acetylcholine receptor

Keith A Krolick, Olgui E. Urso

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.

Original languageEnglish (US)
Pages (from-to)75-87
Number of pages13
JournalJournal of Neuroimmunology
Volume13
Issue number1
DOIs
StatePublished - 1986

Fingerprint

T-Cell Antigen Receptor Specificity
Cholinergic Receptors
Antibody Formation
T-Lymphocytes
B-Lymphocytes
Helper-Inducer T-Lymphocytes
Lymph Nodes
Antibodies
In Vitro Techniques

Keywords

  • Acetylcholine receptor
  • Autoimmunity
  • Myasthenia gravis
  • T cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Influence of T cell specificity on the antibody response to the acetylcholine receptor. / Krolick, Keith A; Urso, Olgui E.

In: Journal of Neuroimmunology, Vol. 13, No. 1, 1986, p. 75-87.

Research output: Contribution to journalArticle

@article{e7e3c7b4b2864af2bef0ba2c7823ea3b,
title = "Influence of T cell specificity on the antibody response to the acetylcholine receptor",
abstract = "Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.",
keywords = "Acetylcholine receptor, Autoimmunity, Myasthenia gravis, T cells",
author = "Krolick, {Keith A} and Urso, {Olgui E.}",
year = "1986",
doi = "10.1016/0165-5728(86)90051-2",
language = "English (US)",
volume = "13",
pages = "75--87",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Influence of T cell specificity on the antibody response to the acetylcholine receptor

AU - Krolick, Keith A

AU - Urso, Olgui E.

PY - 1986

Y1 - 1986

N2 - Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.

AB - Lymph node T cells were obtained from rats immunized with purified acetylcholine receptor (AChR) or isolated receptor subunits (α, β, γ, or σ). The immune T cells were then analyzed for their ability to respond to native AChR or AChR subunit challenge in an in vitro proliferation assay, as well as to perform as helper T cells (TH) in an in vitro anti-AChR antibody response. Results indicated a significant degree of subunit-to-subunit crossreactivity at the T cell level. Moreover, helper function could be generated by stimulation of T cells immune to any of the AChR subunits, although α-immune TH cells appeared to provide a quantifiably increased level of specific antibody production by AChR-immune B cells in vitro when compared to antibody produced by the same B cells in conjunction when β-, γ-, or σ-immune TH cells.

KW - Acetylcholine receptor

KW - Autoimmunity

KW - Myasthenia gravis

KW - T cells

UR - http://www.scopus.com/inward/record.url?scp=0022486820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022486820&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(86)90051-2

DO - 10.1016/0165-5728(86)90051-2

M3 - Article

C2 - 2428835

AN - SCOPUS:0022486820

VL - 13

SP - 75

EP - 87

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1

ER -