Influence of cigarette smoking on prostaglandin synthesis and cyclooxygenase-2 gene expression in human urinary bladder cancer

Alaa F. Badawi, Samy L. Habib, Mohammed A. Mohammed, Ahmed A. Abadi, Michael S. Michael

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The present study examines the effect of tobacco smoking on the expression of cyclooxygenase (COX)-2 gene, COX enzymatic activity and prostaglandin (PG) synthesis in urothelial mucosal tissues from patients with bladder cancer and from normal individuals. The detection frequency of COX-2 mRNA was 2-fold higher in bladder cancer patients compared to controls and it was accompanied by a significantly increased COX enzymatic activity and PGE2 synthesis (p < 0.05). Smokers, in both control and patients groups, had higher COX-2 expression, COX activity, and PGE2 synthesis compared to the nonsmokers (p < 0.05). The number of cigarettes smoked in the cases, but not controls, correlated well with COX enzymatic activity (r = 0.42, p = 0.016). The observedover-expression of COX-2 gene in human urinary bladder and the concomitant increases in PG synthesis may explain, at least in part, the mechanism by which cigarette smoking influences the development of urothelial neoplasia.

Original languageEnglish (US)
Pages (from-to)651-656
Number of pages6
JournalCancer Investigation
Volume20
Issue number5-6
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Keywords

  • Bladder cancer
  • Cyclooxygenase
  • Prostaglandins
  • Smoking

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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